Difference between revisions of "Garcia-Rivas 2013 Abstract MiP2013"
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{{Abstract | {{Abstract | ||
|title=Garcia-Rivas G,Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N(2013) Regulation of mitochondrial permeability transition by Sirt3-catalyzed cyclophilin D deacetylation and its relevance for ventricular dysfunction in metabolic syndrome. Mitochondr Physiol Network 18.08. | |title=Garcia-Rivas G,Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N(2013) Regulation of mitochondrial permeability transition by Sirt3-catalyzed cyclophilin D deacetylation and its relevance for ventricular dysfunction in metabolic syndrome. Mitochondr Physiol Network 18.08. | ||
|info=[http://www.mitophysiology.org/?MiP2013 MiP2013] | |info=[http://www.mitophysiology.org/?MiP2013 MiP2013], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] | ||
|authors=Garcia-Rivas G,Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N | |authors=Garcia-Rivas G,Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N | ||
|year=2013 | |year=2013 |
Revision as of 09:50, 14 September 2013
Garcia-Rivas G,Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N(2013) Regulation of mitochondrial permeability transition by Sirt3-catalyzed cyclophilin D deacetylation and its relevance for ventricular dysfunction in metabolic syndrome. Mitochondr Physiol Network 18.08. |
Link: MiP2013, Book of Abstracts Open Access
Garcia-Rivas G, Morales JA, Vega-Sevilla L, Silva-Platas C, GarcĂa N (2013)
Event: MiP2013 Programme
Metabolic syndrome (MS) can be defined as a group of signs that increases the risk of cardiovascular diseases (CVD). These signs include central obesity, hypertriglyceridemia and hypertension. We are interested in the mechanisms that trigger ventricular dysfunction in a MS murine model, as a way to understand how mitochondrial function fails in CVD. The sustained opening of the mitochondrial permeability transition pore (PTP) is a major event in the onset of irreversible myocardial injury. Several mitochondrial proteins modulate the PTP, including the cyclophilin D (CyD), the adenine nucleotide translocator (ANT) and the SIRTUINS. In this regard, SIRT-3 has emerged recently as a pivotal mediator of mitochondrial metabolism and PTP inductor in a knockout murine model. However, the precise role of SIRT-âą3 in a pathophysiologic context remains indefinable. Male Wistar rats with sucrose-induced MS were subjected to cardiac echocardiography and ex-vivo contraction measurements at 6 and 12 months. Respiratory activity, calcium retention capacity and deacetylation profile were investigated in isolated mitochondria. The expression of SIRT-3, ANT and CyD was evaluated by qPCR or western blot. We observed differences in the E/A ratio (control 1.07±0.01 vs. MS 0.85±0.06, P<0.029) and the ventricular deceleration time (0.029±0.002 vs. 0.034±0.003, P<0.04) indicating an abnormal lusitropism. No significant differences were found in respiratory activity and respiratory control from isolated mitochondria. Nevertheless, calcium retention in MS mitochondria was reduced (0.83±0.03 vs 0.65±0.04 Abs.min-1, P<0.004) indicating premature PTP opening. Proneness in PTP opening was associated with lower expression (60% p<0.001) of SIRT-3. These changes correlated with the MS heartÂŽs mitochondrial acetylation profile. It appears that metabolic changes inherent to MS promote alterations in the expression of SIRT-3 and correlates with PTP opening sensibility.
Labels: MiParea: Respiration Pathology: Cardiovascular, Diabetes, Other Stress:Permeability transition Organism: Rat
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Calcium
MiP2013
Affiliations and author contributions
1 - CĂĄtedra de CardiologĂa y Medicina Vascular. Escuela de Medicina. TecnolĂłgico de Monterrey. MĂ©xico.
2 - Centro de InvestigaciĂłn BĂĄsica y Transferencia. Instituto de CardiologĂa y Medicina Vascular. Tec Salud del Sistema TecnolĂłgico de Monterrey. San Pedro Garza GarcĂa. MĂ©xico.
Email: [email protected]