Boushel 2011 Mitochondrion: Difference between revisions
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|year=2011 | |year=2011 | ||
|journal=Mitochondrion | |journal=Mitochondrion | ||
|abstract=Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in | |abstract=Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely ''in vivo'' maximal oxygen consumption (''V''O(2)max) is matched to muscle tissue-specific OXPHOS capacity ([[State 3]]) respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while ''in vivo'' arm and leg ''V''O(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3ยฑ0.4 mmol O(2)kg(-1)min(-1)) was similar to the ''in vivo'' ''V''O(2) during maximal arm cranking (4.7ยฑ0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9ยฑ0.5 mmol O(2)kg(-1)min(-1), exceeding the ''in vivo'' leg ''V''O(2)max (5.0ยฑ0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (''P''<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses ''in vivo'' ''V''O(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans. | ||
|mipnetlab=SE Stockholm Boushel RC, AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom | |mipnetlab=SE Stockholm Boushel RC, AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology |
Revision as of 14:48, 2 July 2015
Boushel RC, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11:303-7. |
Boushel RC, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Mitochondrion
Abstract: Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in vivo maximal oxygen consumption (VO(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3) respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in vivo arm and leg VO(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3ยฑ0.4 mmol O(2)kg(-1)min(-1)) was similar to the in vivo VO(2) during maximal arm cranking (4.7ยฑ0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9ยฑ0.5 mmol O(2)kg(-1)min(-1), exceeding the in vivo leg VO(2)max (5.0ยฑ0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (P<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in vivo VO(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.
โข O2k-Network Lab: SE Stockholm Boushel RC, AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
Stress:Ischemia-reperfusion Organism: Human Tissue;cell: Skeletal muscle Preparation: Intact organism, Permeabilized tissue
Coupling state: OXPHOS
HRR: Oxygraph-2k