Description
Pyruvic acid, C3H4O3, is an alpha-keto acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate as an anion that enters the mitochondrial matrix via a specific low Km' transporter known as the pyruvate carrier. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.
Abbreviation: P
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 Β°C; FW = 110.0
Preparation of 2 M stock solution (dissolved in H2O)
- Prepare fresh everyday.
- 1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
- 2) Add 0.2 ml H2O.
- 3) Adjust pH to 7.0.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 Β΅l using a 25 Β΅l syringe (2 ml O2k-chamber).
- Final concentration: 5 mM.