Pyruvate: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=P | |abbr=P | ||
|description= | |description=[[File:Pyruvic_acid.jpg|left|100px|Pyruvic acid]] | ||
In the mt matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields acetyl-CoA. | '''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, M''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate as an anion that enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' transporter known as the [[pyruvate carrier]]. | ||
In the mt-matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields [[acetyl-CoA]]. | |||
Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by cyanide. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]]. | |||
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]] | |info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]] | ||
|type=Substrate ETS | |type=Substrate ETS | ||
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'''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 ยฐC; FW = 110.0 | '''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 ยฐC; FW = 110.0 | ||
'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O) | ||
:: Prepare fresh everyday. | |||
::1) | ::1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube. | ||
::2) | ::2) Add 0.2 ml H<sub>2</sub>O. | ||
::3) | ::3) Adjust pH to 7.0. | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 5 ยตl using a 25 ยตl syringe | ::* Titration volume: 5 ยตl using a 25 ยตl syringe (2 ml O2k-chamber). | ||
::* Final | ::* Final concentration: 5 mM. |
Revision as of 09:25, 15 April 2014
Description
Pyruvic acid, C3H4O3, is an alpha-keto acid which occurs under physiological conditions mainly as the anion pyruvate-, M, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate as an anion that enters the mitochondrial matrix via a specific low Km' transporter known as the pyruvate carrier. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.
Abbreviation: P
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 ยฐC; FW = 110.0
Preparation of 2 M stock solution (dissolved in H2O)
- Prepare fresh everyday.
- 1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
- 2) Add 0.2 ml H2O.
- 3) Adjust pH to 7.0.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 ยตl using a 25 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 5 mM.