Myxothiazol: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Myx | |abbr=Myx | ||
|description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII inhibits | |description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor |
Revision as of 14:54, 17 October 2016
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 ยฐC: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 ml ethanol.
- Divide into 0.2 ml (use glass vials).
- Store at -20 ยฐC.
Oxygraph-2k manual titrations MiPNet09.12
- Titration volume: 1 ยตl using a 10 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 ยตM.
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.