Difference between revisions of "Woodhead 2020 J Appl Physiol (1985)"
Β |
|||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL (2020) High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men. J Appl Physiol (1985) | |title=Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL (2020) High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men. J Appl Physiol (1985) 128:1346-54. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/32271093 PMID: 32271093] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/32271093 PMID: 32271093] | ||
|authors=Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL | |authors=Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL | ||
Line 11: | Line 11: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, mtDNA;mt-genetics, Exercise physiology;nutrition;life style | ||
|organism=Human | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue | |||
|couplingstates=OXPHOS | |||
|pathways=N, NS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2020-04 | |additional=2020-04 | ||
}} | }} |
Latest revision as of 17:53, 1 July 2020
Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL (2020) High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men. J Appl Physiol (1985) 128:1346-54. |
Woodhead JST, D'Souza RF, Hedges CP, Wan J, Berridge MV, Cameron-Smith D, Cohen P, Hickey AJR, Mitchell CJ, Merry TL (2020) J Appl Physiol (1985)
Abstract: Humanin, a small regulatory peptide encoded within the 16S ribosomal RNA gene (MT-RNR2) of the mitochondrial genome, has cellular cyto- and metabolo-protective properties similar to that of aerobic exercise training. Here we investigated whether acute high-intensity interval exercise or short-term high-intensity interval training (HIIT) impacted skeletal muscle and plasma humanin levels. Vastus lateralis muscle biopsies and plasma samples were collected from young healthy untrained men (n=10, 24.5Β±3.7 y) before, immediately following, and 4 h following the completion of 10 x 60 s cycle ergometer bouts at VO2peak power output (untrained). Resting and post-exercise sampling was also performed after six HIIT sessions (trained) completed over 2 weeks. Humanin protein abundance in muscle and plasma were increased following an acute high-intensity exercise bout. HIIT trended (p=0.063) to lower absolute humanin plasma levels, without effecting the response in muscle or plasma to acute exercise. A similar response in the plasma was observed for the small humanin-like peptide 6 (SHLP6), but not SHLP2, indicating selective regulation of peptides encoded by MT-RNR2 gene. There was a weak positive correlation between muscle and plasma humanin levels, and contraction of isolated mouse EDL muscle increased humanin levels approximately 4-fold. The increase in muscle humanin levels with acute exercise was not associated with MT-RNR2 mRNA or humanin mRNA levels (which decreased following acute exercise). Overall, these results suggest that humanin is an exercise sensitive mitochondrial peptide and acute exercise-induced humanin responses in muscle are non-transcriptionally regulated and may partially contribute to the observed increase in plasma concentrations. β’ Keywords: Exercise, Mitochondrial derived peptides, Mitokine, Muscle, Small humanin-like peptides β’ Bioblast editor: Plangger M β’ O2k-Network Lab: NZ Auckland Hickey AJ
Labels: MiParea: Respiration, mtDNA;mt-genetics, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: OXPHOS
Pathway: N, NS
HRR: Oxygraph-2k
2020-04