Varikmaa 2014 Biochim Biophys Acta
Varikmaa M, Bagur R, Kaambre T, Grichine A, Timohhina N, Tepp K, Shevchuk I, Chekulayev V, Metsis M, Boucher F, Saks V, Kuznetsov AV, Guzun R (2013) Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles. Biochim Biophys Acta [Epub ahead of print]. |
Varikmaa M, Bagur R, Kaambre T, Grichine A, Timohhina N, Tepp K, Shevchuk I, Chekulayev V, Metsis M, Boucher F, Saks V, Kuznetsov AV, Guzun R (2013) Biochim Biophys Acta
Abstract: The aim of this work was to study the regulation of respiration and energy fluxes in permeabilized oxidative and glycolytic skeletal muscle fibers, focusing also on the role of cytoskeletal protein tubulin Ξ²II isotype in mitochondrial metabolism and organization. By analyzing accessibility of mitochondrial ADP, using respirometry and pyruvate kinase-phosphoenolpyruvate trapping system for ADP, we show that the apparent affinity of respiration for ADP can be directly linked to the permeability of the mitochondrial outer membrane (MOM). Previous studies have shown that MOM permeability in cardiomyocytes can be regulated by VDAC interaction with cytoskeletal protein, Ξ²II tubulin. We found that in oxidative soleus skeletal muscle the high apparent Km for ADP is associated with low MOM permeability and high expression of non-polymerized Ξ²II tubulin. Very low expression of non-polymerized form of Ξ²II tubulin in glycolytic muscles is associated with high MOM permeability for adenine nucleotides (low apparent Km for ADP). β’ Keywords: Energy flux, Intracellular energy unit, Metabolic control analysis, Respiration, Skeletal muscle
β’ O2k-Network Lab: FR Grenoble Saks VA, FR Grenoble Schlattner U
Labels: MiParea: Respiration, mt-Membrane, Comparative MiP;environmental MiP
Organism: Rat
Tissue;cell: Heart, Skeletal muscle
Preparation: Permeabilized cells, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Inhibitor, PCr;Cr Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k