Difference between revisions of "Tissue homogenate"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=n.a. | ||
|description=A '''tissue homogenate''' is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized. Β | |description=A '''tissue homogenate''' is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized. | ||
|info=[[MiPNet17.02 PBI-Shredder-Manual]] | |info=[[MiPNet17.02 PBI-Shredder-Manual]] | ||
|type=Method | |||
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{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
|type=Method | |type=Method | ||
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{{MitoPedia topics|type=Method | {{MitoPedia topics|type=Method | ||
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Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration ([[Pecinova 2011 Mitochondrion]]). | Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration ([[Pecinova 2011 Mitochondrion]]). |
Revision as of 04:49, 27 August 2013
Description
A tissue homogenate is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized.
Abbreviation: n.a.
Reference: MiPNet17.02 PBI-Shredder-Manual
MitoPedia methods:
Respirometry
Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration (Pecinova 2011 Mitochondrion).