Difference between revisions of "Template talk:Next oroboros ecosystem agenda"
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==Ā O2k-Open Support alert == | ==Ā O2k-Open Support alert == |
Revision as of 14:20, 1 September 2021
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- Ā»Instrumental backgroundĀ« and Ā»TIP2k Filter PapersĀ«
- See the scheme:
Bioblast alert 2021
Prep for circular:
For Prostate Cancer Bioblast Circular (September?):
Succinate anaplerosis enhances the malignant potential of prostate cancer cells.
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»FR Bordeaux Rossignol RĀ«
- Bastos Sant'Anna Silva AC, Perez-Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. Cancers 13:1727.Ā»Bioblast linkĀ«
pH-regulated succinate uptake in prostate cancer
- from the O2k-Network Ā»US PA Philadelphia Orynbayeva ZĀ«
- Zhunussova A, Sen B, Friedman L, Tuleukhanov S, Brooks AD, Sensenig R, Orynbayeva Z (2015) Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria. Am J Cancer Res 5:1665-79. Ā»Bioblast linkĀ«
Succinate: a key regulator of mitochondrial function and HIF-1Ī± upregulation in prostate cancer
- from the O2k-Network Ā»AT Innsbruck OroborosĀ«, Ā»AT Innsbruck Gnaiger EĀ«
- Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1Ī± upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. Ā»Bioblast linkĀ«
Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»AT Innsbruck OroborosĀ«
- Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, SantāAnna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. Ā»Bioblast linkĀ«
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»AT Innsbruck OroborosĀ«
MitoPedia alert
Next
O2k-Brief alert
Next
O2k-Publications alert
Author names of O2k-Publications/ Brief for the Agenda vs Bioblast
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
- Example: Oroboros Ecosystem Agenda example
- This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
- 2.Bioblast: SOP: [1] ā examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Guidelines to update a reference
- When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:
- Correct format for a person called āFirst Name Surnameā:
- In the gray box should be āSurname FNā - From where references to be used as O2k-Publication/Brief alerts are copied from.
- In the links should be ā Surname First Nā
- How to edit:
- A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps āHow to move a profileā below.
- B) Click in āEdit with formā.
- C) The gray box is edited in āReferenceā and the links are changed in āAuthor(s)ā. Change it accordingly and click in āsave pageā at the end of the page.
- How to edit:
How to move a profile (instructions sent by Marija):
So letās take for example my page: Beno M [2]
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 1. Go to e.g. āBeno Mā on edit
- 2. Click on āMoveā
- 3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 4. Now it should look like that:
- 5. Click on āMoveā and leave all the ticked boxes
- 6.Done
- After that you can continue in step B.
- If you have any doubt you can contact Cristiane, Marija or Mario.
Next
O2k-Open Support alert
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Next
- Month-##
When filling in the volume of uncoupler in the DatLab mark or the excel template, should one enter the total volume added or the volume to get to the highest flux?
The volume added until the mark, equal to the highest flux, should be entered. Anything titrated after this mark should be summed to the other volumes before the next mark because this will be considered to calculate the sample dilution.
- Ā»LINK and O2 flux analysis with DatLab 7.4Ā«
- communicated by Ā»Cardoso LuizaĀ« and Ā»[[ ]]Ā«
Under discussion
Removed for correction
- Nov-30 Mo
Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections.
Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol.
- Ā»Cleaning the electrical connectionsĀ«
- communicated by Ā»Schmitt SabineĀ« and Ā»Komlodi TimeaĀ«
WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- Ā»Leaky chamber testĀ« and Ā» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assemblyĀ«
O2k-Open Support alert circulars
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 Ā°C solid stability for more than 3 years (3 years 7 months)
- Proven -20 Ā°C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 Ā°C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1
Postponed events
- Ā»36th Congress Czech Nutrition Society 2020 Hradec Kralove CZĀ« (Sep 10-12)