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Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
Using different fluorescence sensors ([[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or [[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set|Selecting a Filter Set]]'''«
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set|Selecting a Filter Set]'''«
|}
|}



Revision as of 17:30, 29 July 2020

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format


Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

  • Aug-06 Th
MitoPedia
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system, and is the ratio of free to total OXPHOS capacity. It is suggested to use instead of respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.

- »OXPHOS-coupling efficiency«

Other suggestions:

MitoPedia
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system. This coupling control factor ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (L=P). It is suggested to use OXPHOS-coupling efficiency instead of the respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.

- »OXPHOS-coupling efficiency«

MitoPedia
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system. This coupling control factor has boundaries from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (L=P). It is suggested to use OXPHOS-coupling efficiency instead of the respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.

- »OXPHOS-coupling efficiency«

  • Aug-13 Th
MitoPedia
Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration.

- »Photorespiration«

O2k-Brief alert

  • Aug-14 Fr
O2k-brief repository Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis
from the O2k-Network »NZ Auckland Hickey AJ« 
  • Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. »Bioblast link«

Next

O2k-Publications alert

Next

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
  • Aug-10 Mo

Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(07): in prep

MitoPedia
Do you know how to select the proper Fluorescence-Sensor and filter set?

Using different Fluorescence-Sensors and filters, the O2k-Fluo Smart-Module allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Setup of the O2k-Fluo LED2-Module«

  • Comments by Caro:
  • Suggestion:
MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors (with the O2k-Fluo Smart-Module or with the LED2-Module) and LED and photodiode filters, the O2k-Fluo Smart-Module allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »a Filter Set«


MitoPedia
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

MitoPedia
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?

- »Incompatible substrates«

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US