Template:Select fluorescence sensor: Difference between revisions
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:::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | :::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | ||
:::: Select the Fluo-Sensor and filter set from the table | :::: Select the Fluo-Sensor and filter set from the table below in the section "Fluorescence dyes and settings". Each Fluo-Sensor is delivered with a mounted filter set: ย | ||
::::# [[Fluorescence-Sensor Green]] and [[Smart Fluo-Sensor Green]]: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H<sub>2</sub>O<sub>2</sub> measurement with [[Amplex red|Amplexยฎ UltraRed]] or for measurements of mt-membrane potential with [[TMRM]]. | ::::# [[Fluorescence-Sensor Green]] and [[Smart Fluo-Sensor Green]]: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H<sub>2</sub>O<sub>2</sub> measurement with [[Amplex red|Amplexยฎ UltraRed]] or for measurements of mt-membrane potential with [[TMRM]]. | ||
::::# [[Fluorescence-Sensor Blue]] and [[Smart Fluo-Sensor Blue]]: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]] or [[Rhodamine 123]]. A different filter is used for measurement with [[Magnesium green|Magnesium Greenโข]] or Calcium Greenโข. | ::::# [[Fluorescence-Sensor Blue]] and [[Smart Fluo-Sensor Blue]]: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]] or [[Rhodamine 123]]. A different filter is used for measurement with [[Magnesium green|Magnesium Greenโข]] or Calcium Greenโข. | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor] | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor] |
Latest revision as of 16:23, 23 November 2021
- Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
- Select the Fluo-Sensor and filter set from the table below in the section "Fluorescence dyes and settings". Each Fluo-Sensor is delivered with a mounted filter set:
- Fluorescence-Sensor Green and Smart Fluo-Sensor Green: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H2O2 measurement with Amplexยฎ UltraRed or for measurements of mt-membrane potential with TMRM.
- Fluorescence-Sensor Blue and Smart Fluo-Sensor Blue: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin or Rhodamine 123. A different filter is used for measurement with Magnesium Greenโข or Calcium Greenโข.