Template:SUIT-003 O2 ce D038: Difference between revisions
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[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File:Ce1; | [[File:Ce1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.png|350px]] | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
{{Template:SUIT header}} | {{Template:SUIT header}} | ||
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|- | |- | ||
| | | (ce2Omy) | ||
| ''[[LEAK respiration|L]]''(Omy) | |||
| | |||
| | |||
| ce1;(ce2Omy) | |||
*{{Template: SUIT L(Omy)}} | |||
* If the aim is to analyse only Crabtree effect, it is recommended to skip this step. However, if there is also interest in studying coupling control, Omy can be added at this point to reach LEAK. | |||
ย | |||
|- | |||
| ce3U | |||
| ''[[E]]'' | | ''[[E]]'' | ||
| ย | | ย | ||
| | | | ||
| ce1; | | ce1;(ce2Omy);ce3U | ||
*{{Template: SUIT ET}} | *{{Template: SUIT ET}} | ||
Line 26: | Line 35: | ||
| ย | | ย | ||
| ย | | ย | ||
| ce1; | | ce1;(ce2Omy);ce3U;ce3Glc | ||
*{{Template:SUIT ET}} ย | *{{Template:SUIT ET}} | ||
*{{Template:SUIT Glc}} | |||
ย | |||
|- | |||
| ce3'U | |||
| ''[[E]]'' | |||
| | |||
| | |||
| ce1;(ce2Omy);ce3U;ce3Glc;ce3'U | |||
*{{Template: SUIT ET}} | |||
* Additional uncoupler titrations are performed to ensure reaching ET state after addition of glucose. | |||
|- | |- | ||
| | | ce4Ama | ||
| | | [[ROX]] | ||
| ย | | ย | ||
| | | | ||
| ce1; | | ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama | ||
*{{Template:SUIT ROX}} | *{{Template:SUIT ROX}} | ||
|} | |} | ||
{{ | {{Keywords: SUIT protocols}} |
Latest revision as of 13:31, 25 November 2020
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
(ce2Omy) | L(Omy) | ce1;(ce2Omy)
| ||
ce3U | E | ce1;(ce2Omy);ce3U
| ||
ce3Glc | E | ce1;(ce2Omy);ce3U;ce3Glc
| ||
ce3'U | E | ce1;(ce2Omy);ce3U;ce3Glc;ce3'U
| ||
ce4Ama | ROX | ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary