Template:MitoPediaAlert2024: Difference between revisions
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:::::: <big>''A collection from the »[[OROBOROS_INSTRUMENTS|Bioblast Agenda]]«</big> | :::::: <big>''A collection from the »[[OROBOROS_INSTRUMENTS|Bioblast Agenda]]«</big> | ||
__TOC__ | __TOC__ | ||
== Mar 2024 == | |||
* '''Mar-11 Mo''' | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | |||
|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br> | |||
Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered. <br> | |||
- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br> | |||
- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]« | |||
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== Feb 2024 == | == Feb 2024 == | ||
* '''Feb-26 Mo''' | * '''Feb-26 Mo''' | ||
Revision as of 10:59, 11 March 2024
- A collection from the »Bioblast Agenda«
Mar 2024
- Mar-11 Mo
Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O2] regime?
Long diffusion distances in the pfi result in an O2 diffusion limitation, which is overcome by increasing the O2 partial pressure inside the chamber. Proper instrumental background correction is a must and O2 dependence of ROS production should be considered.
- »Permeabilized muscle fibers«, »Oxygen flux - instrumental background«
- communicated by »Grings Mateus« and »Cardoso Luiza«
Feb 2024
- Feb-26 Mo
ATP synthase = phosphorylation system?
No, the phosphorylation system consists of adenine nucleotide translocase, phosphate carrier, and ATP synthase, which carry out the steps in the phosphorylation pathway.
- »Phosphorylation pathway«, »Mitochondrial Pathways: Chapter 2.2«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«
- Feb-12 Mo
What is the O2 solubility factor of the medium (FM) and why is it important?
FM expresses the effect of the salt concentration on oxygen solubility relative to pure water, and varies depending on the temperature and composition of the medium. The correct FM needs to be entered in DatLab when performing an air calibration.
- »Oxygen solubility factor« , »Air calibration in DatLab«
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«
Jan 2024
- Jan-31 We
Can the S-pathway O2 flux be derived by subtracting N-pathway O2 flux from NS-pathway O2 flux?
No, the sum of the O2 fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity:
- »Additive effect of convergent electron flow«
- communicated by »Cardoso Luiza« and »Grings Mateus«
- Jan-02 Tu
New year, new state? What is REN state?
REN state is now distinguished from ROX state: REN is measured in mitochondrial preparations without addition of substrates, whereas ROX is due to oxidative side reactions remaining after inhibition of the electron transfer pathway.
- »REN«, »ROX«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«
