MitoPediaAlert2020 Archive
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
June 2020
- Jun-04 Th
Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration?
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-»SUIT-008« and »SUIT-014«
Previous MitoPedia Alert 2020
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May 2020
- May-28 Th
Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas?
- May-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production.
-»PhotoBiology«
- May-14 Th
How can I measure ATP production with the O2k?
Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- »Magnesium Green«
- May-07 Th
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Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«
Apr 2020
- Apr-24 Fr
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ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
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Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
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Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«
- Apr-08 We
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The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«
- Apr-02 Th
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Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
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The standardized coupling control protocol to explore the bioenergetics of living cells
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«
- Mar-11 We
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Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.
- »Blebbistatin«
- Mar-06 Fr
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Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
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Rapamycin - more differences or more in common with other mitObesity drugs?
- »Rapamycin«
Feb 2020
- Feb-26 We
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It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Feb-24 Mo
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More research is needed on the impact of viral infection on mitochondrial function.
- Feb-19 We
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Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.
- »Spermidine«
- Feb-18 Tu
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Why do we fight gas bubbles?
- Dive deep into »Oxygen solubility«
- Feb-12 We
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What do metformin, melatonin and other bioactive mitObesity compounds have in common?
- »MitObesity drugs«
Jan 2020
- Jan-16 Th
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Oxygen flux
- which SI units should be used?
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Did you ever think that units for body mass could be an issue?
- »Body mass«
- Jan-08 We
