Template:Fluorescence dyes and settings: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
| [[Amplex UltraRed|Amplex<sup>TM</sup> UltraRed]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || [[O2k_signal_and_output|Type II]] || 100 - 500 || 1000 || at c(AmR) = 10 µM | | [[Amplex UltraRed|Amplex<sup>TM</sup> UltraRed]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || [[O2k_signal_and_output|Type II]] || 100 - 500 || 1000 || at c(AmR) = 10 µM | ||
|- | |- | ||
| [[TMRM]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || [[O2k_signal_and_output|Type III]] || | | [[TMRM]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || [[O2k_signal_and_output|Type III]] || 100 - 500|| 1000 || at c(TMRM) = 2 µM | ||
|- | |- | ||
| [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] || [[O2k_signal_and_output| Type III]] || 100 - | | [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] || [[O2k_signal_and_output| Type III]] || 100 - 250 || 1000 || at c(safranin) = 2 µM | ||
|- | |||
| [[Rhodamine 123]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] || [[O2k_signal_and_output| Type III]] || 100 - 250 || 1000 || at c(Rh123) = 1 µM | |||
|- | |- | ||
| [[Magnesium green|Magnesium Green™]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] || [[O2k_signal_and_output| Type I and II]] || 100 - 500|| 1000 || at c(Mg Green) = 2 µM | | [[Magnesium green|Magnesium Green™]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] || [[O2k_signal_and_output| Type I and II]] || 100 - 500|| 1000 || at c(Mg Green) = 2 µM | ||
Revision as of 15:29, 18 November 2021
| Application | Sensor | Filter set | O2k output | Light intensity (Amp polarization voltage) | Gain | Comment |
|---|---|---|---|---|---|---|
| AmplexTM UltraRed | Fluorescence-Sensor Green | AmR | Type II | 100 - 500 | 1000 | at c(AmR) = 10 µM |
| TMRM | Fluorescence-Sensor Green | AmR | Type III | 100 - 500 | 1000 | at c(TMRM) = 2 µM |
| Safranin | Fluorescence-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(safranin) = 2 µM |
| Rhodamine 123 | Fluorescence-Sensor Blue | Saf | Type III | 100 - 250 | 1000 | at c(Rh123) = 1 µM |
| Magnesium Green™ | Fluorescence-Sensor Blue | MgG / CaG | Type I and II | 100 - 500 | 1000 | at c(Mg Green) = 2 µM |
| Calcium Green™ | Fluorescence-Sensor Blue | MgG / CaG | Type I | 300 - 500 | 1000 | at c(Ca Green) = 2 µM |
In the O2k-LED2-Module (Series D-G) polarization voltage is used, while in the O2k-Fluo Smart Module (Series H-I) fluorescence intensity. To use the same setting in the O2k-Fluo LED2-Module as in the O2k-Fluo Smart-Module use the following Excel sheet:
 Download the EXCEL calculation-sheet to adjust fluorescence intensity |
