Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 12
+Week 21
-* '''Mar-18 Mo'''
+* '''May-20 Mo'''
-:::: '''»[[EU-METAHEART MC2 Meeting 2024 Antalya TR]]«''' Mar 18-21
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
 ----
-* '''Mar-19 Tu'''
+* '''May-21 Tu'''
-:::: 15:05 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' - ''[[Donnelly_2024_EU-METAHEART_MC2_Meeting |Functional hypoxia in cardiac mitochondria: effects on oxidative phosphorylation, mitochondrial membrane potential, redox state of coenzyme Q, and calcium uptake]]''
 ----
-* '''Mar-20 We'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' What if ''Alert: Error in titrations!'' appears in your O2 analysis template file?  '''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.<br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-- »'''[[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|O<sub>2</sub> flux analysis]]'''«<br>
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Mar-21 Th'''
+* '''May-23 Th'''
-:::: '''»[[Club17 2024 Innsbruck AT]]«'''
-:::: '''17:00 [[Laner Verena|Verena Laner]] presenting at [[Club17 2024 Innsbruck AT]]''' - ''[[Functional Mitochondrial Diagnostics]]''
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How well do you handle your Hamilton syringes? What is a cleaning cycle?'''<br>
-"A syringe cleaning cycle consists of (1) filling an empty syringe with a washing solution while immersing [both the needle and] the base of the barrel into the washing solution, without fully removing the plunger, and (2) emptying the syringe into the waste beaker." <br>
-- »'''[[MiPNet19.14 SOP Hamilton microsyringes]]'''«<br>
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
-|}
 ----
-* '''Mar-22 Fr'''
+* '''May-24 Fr'''
 ----
-Week 13
+'''<big>Next Week</big>'''
+Week 22
-* '''Mar-25 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Living cells or intact cells? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Living cells]]'''«, »'''[[BEC 2020.1]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
+<br>
-- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Mar-26 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Mar-27 We'''
+* '''May-29 We'''
 ----
-* '''Mar-28 Th'''
+* '''May-30 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »'''[[O2k-Feedback]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Mar-29 Fr'''
+* '''May-31 Fr'''
+----