Template:Current week ecosystem agenda: Difference between revisions

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
  2024 Week 05
 
* '''Jan-29 Mo'''
2024 Week 18
:::: '''»[[E-mit Winter School 2024 Brixen IT]]«''' Jan 28 - Feb 02
 
* '''Apr-29 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can I update DatLab 8 to the latest version?'''<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
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* '''Jan-30 Tu'''
* '''Apr-30 Tu'''  
:::: '''16:30  [[Gnaiger Erich|Erich Gnaiger]] presenting at [[E-mit Winter School 2024 Brixen IT]]''' - ''Coupling control and pathway control in mitochondrial high-resolution respirometry''
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* '''Jan-31 We'''  
* '''May-01 We'''  
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Can the S-pathway O<sub><small>2</small></sub> flux be derived by subtracting N-pathway O<sub><small>2</small></sub> flux from NS-pathway O<sub><small>2</small></sub> flux?'''<br>
No, the sum of the O<sub><small>2</small></sub> fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity: <br>
- »'''[[Additive effect of convergent electron flow]]'''« <br>
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
|}
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* '''Feb-01 Th'''  
* '''May-02 Th'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' ''“Doing the entire hands-on module from start to finish was the best for really “cementing” theory and hands-on application together.”'''''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
[[Cosgrove Richard]] from Eli Lilly Research Labs, on what he liked best on [[IOC162]]<br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- »'''[[O2k-Feedback]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''Feb-02 Fr'''  
* '''May-03 Fr'''
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2024 Week 06
  2024 Week 19


* '''Feb-05 Mo'''
* '''May-06 Mo'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]]
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
:from the O2k-Network »[[SK Bratislava Sumbalova Z]]«
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Feb-06 Tu'''  
* '''May-07 Tu'''  
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* '''Feb-07 We'''  
* '''May-08 We'''  
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* '''Feb-08 Th'''  
* '''May-09 Th'''  
 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br>
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
"For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration."<br>
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-»'''[[MiPNet09.12 O2k-Titrations]]'''«<br>
:from the O2k-Network »[[IT Padova Morosinotto T]]«
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
|}
|}
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* '''May-10 Fr'''
* '''Feb-09 Fr'''  
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Revision as of 12:50, 2 May 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«
  • May-10 Fr
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