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Revision as of 12:50, 2 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

@@ Line 1: / Line 1: @@
-Week 31
 {{Template:Science for Ukraine}}
-*'''Aug-01 Mo'''
-[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
+Week 18
+* '''Apr-29 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' O2k-checklist: Get started with an O2k-experiment''' <br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-<br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- » '''[[MiPNet20.04_O2k-checklist]]'''« <br>
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-- » communicated by »[[Cecatto Cristiane]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Aug-02 Tu'''
+* '''Apr-30 Tu'''
-[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
+----
-:{|
+* '''May-01 We'''
-| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
-|''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br>
-These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
-<br>
-- »''' [[SUIT reference protocol]]'''«
-- communicated by »[[Cardoso Luiza HD]]« and »[[Tindle-Solomon Lisa]]«
-|}
 ----
-*'''Aug-03 We'''
+* '''May-02 Th'''
-[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
 :{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-|''' O<sub>2</sub> flux analysis with DatLab 7.4'''<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
-Use the O<sub>2</sub> analysis template:   <br>
--  »'''[https://www.youtube.com/watch?v=HRAGvmaK01I%7C O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br>
 |}
 ----
+* '''May-03 Fr'''
-*'''Aug-04 Th'''
+'''<big>Next Week</big>'''
-[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
+Week 19
+* '''May-06 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-|''' Why do we perform multiple H<sub>2</sub>O<sub>2</sub> calibrations in the Amplex® UltraRed assay?'''<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps.  <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- »'''[[Amplex UltraRed]]'''« and  »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodi et al BEC 2021.4]]'''«<br>
 |}
 ----
+* '''May-07 Tu'''
-*'''Aug-05 Fr'''
+----
-[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
+* '''May-08 We'''
+----
+* '''May-09 Th'''
 :{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-|'''Watch these videos to get started with the Q-Module'''<br>
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
-- »'''[https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport:Q-Module]'''«<br>
 |}
+* '''May-10 Fr'''