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Revision as of 12:50, 2 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

@@ Line 1: / Line 1: @@
-Week 42
+{{Template:Science for Ukraine}}
-* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
+Week 18
+* '''Apr-29 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Oct-13 Tu'''
+* '''Apr-30 Tu'''
-:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
+----
+* '''May-01 We'''
 ----
-* '''Oct-14 We'''
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Oct-15 Th'''
+* '''May-03 Fr'''
+'''<big>Next Week</big>'''
+Week 19
+* '''May-06 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- »'''[[O2k-sV-Module]]'''«  <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Oct-16 Fr'''
+* '''May-07 Tu'''
+----
+* '''May-08 We'''
+----
+* '''May-09 Th'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-| '''Which SUIT protocol can you use to evaluate mitochondrial respiration of [[PBMC]] and [[Platelet|platelets]]?'''<br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-The following SUIT protocols will guide you through the titration experiments for these cell models <br>
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-- »'''[[SUIT-001 O2 ce-pce D004]]'''« and »'''[[SUIT-002 O2 ce-pce D007a]]'''« <br>
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
 |}
+* '''May-10 Fr'''
-----
-* '''Oct-17 Sa'''
-----
-* '''Oct-18 Su'''
-:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«