Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:50, 2 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

@@ Line 1: / Line 1: @@
-Week 37
+{{Template:Science for Ukraine}}
-* '''Sep-07 Mo'''
+Week 18
+* '''Apr-29 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-| '''Why it is necessary to perform multiple H<sub>2</sub>O<sub>2</sub> calibrations when using the [[Amplex UltraRed]] (AmR) assay?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-The sensitivity to H<sub>2</sub>O<sub>2</sub> of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H<sub>2</sub>O<sub>2</sub> calibration before sample addition, after sample addition and multiple times during the experiment.  <br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-- »[[Komlodi 2018 Methods Mol Biol]]« <br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H<sub>2</sub>O<sub>2</sub> calibrations implemented. <br>
-- » [https://www.bioblast.at/index.php/SUITbrowser SUITbrowser]« <br>
-- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
 |}
 ----
-* '''Sep-08 Tu'''
+* '''Apr-30 Tu'''
+----
+* '''May-01 We'''
+----
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung'''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-:from the O2k-Network »[[DE Giessen Weissmann N]]«, »[[ FI Tampere Dufour E]]«, »[[FI Helsinki Jacobs HT]]« and »[[DE Jena Szibor M]]«
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. [[Sommer 2020 Sci Adv|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Sep-09 We'''
+* '''May-03 Fr'''
+'''<big>Next Week</big>'''
+Week 19
-----
+* '''May-06 Mo'''
-* '''Sep-10 Th'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P<sub>i</sub>, fuel substrates and O<sub>2</sub>. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of [[excess E-P capacity]] due to kinetic limitations of the phosphorylation system. <br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- »'''[[BEC_2020.1_doi10.26124bec2020-0001.v1|BEC: Mitochondrial physiology]]'''« - Table 3 and several figures. <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- communicated by » [[Cecatto Cristiane]] « and » [[Komlodi Timea]] «
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Sep-11 Fr'''
+* '''May-07 Tu'''
+----
+* '''May-08 We'''
+----
+* '''May-09 Th'''
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
+|}
+* '''May-10 Fr'''