Template:Current week ecosystem agenda: Difference between revisions

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{{Template:Science for Ukraine}}
2020 Week 35


* '''Aug-24 Mo'''  
2024 Week 18
 
* '''Apr-29 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can I update DatLab 8 to the latest version?'''<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
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* '''Apr-30 Tu'''
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* '''May-01 We'''   
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* '''May-02 Th'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Can NADH be directly used as substrate to obtain the NADH electron transfer-pathway state in high-resolution respirometry (HRR)? '''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for [[Mitochondrial_respiration |mitochondrial respiration]] since the [[mitochondrial inner membrane]], which should be intact for HRR experiments, is not permeable to NADH. <br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- »[[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide]]« and »[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]«  
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''Aug-25 Tu'''
* '''May-03 Fr'''


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'''<big>Next Week</big>'''
* '''Aug-26 We'''  
2024 Week 19


* '''May-06 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| '''Non-mitochondrial respiration or [[residual oxygen consumption]] (''Rox'')? — Concept-driven constructive terminology frames our perception.'''  
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
After inhibition of electron-transfer (ET) pathways or in the absence of fuel substrates, both ''mitochondrial'' non-ET reactions and ''non-mitochondrial'' reactions contribute to ''residual oxygen consumption'' in mt-preparations and living cells. <br>
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''« — Table 1: ''Rox'' induced by non-ET pathway oxidation reactions
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
|}
|}
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* '''Aug-27 Th'''  
* '''May-07 Tu'''  
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)
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* '''May-08 We'''
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* '''Aug-28 Fr'''
* '''May-09 Th'''   
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
:from the O2k-Network »[[IT Padova Morosinotto T]]«
|}
* '''May-10 Fr'''

Revision as of 12:50, 2 May 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«
  • May-10 Fr
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