Template:Current week ecosystem agenda: Difference between revisions

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* '''May-04 Mo'''
{{Template:Science for Ukraine}}
 
2024 Week 18
 
* '''Apr-29 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?'''<br>
|'''How can I update DatLab 8 to the latest version?'''<br>
See how to perform the instrumental background test.<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''May-05 Tu'''  
* '''Apr-30 Tu'''  
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse'''
 
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«  »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
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* '''May-06 We'''  
* '''May-01 We'''  
 
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* '''May-07 Th'''
* '''May-02 Th'''  
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
|}
|}
<br />
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* '''May-03 Fr'''


'''<big>Next Week</big>'''
2024 Week 19
* '''May-06 Mo'''
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
|}
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* '''May-07 Tu'''
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* '''May-08 We'''
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* '''May-08 Fr'''
* '''May-09 Th'''   
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
:from the O2k-Network »[[IT Padova Morosinotto T]]«
|}
* '''May-10 Fr'''

Revision as of 12:50, 2 May 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«
  • May-10 Fr
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