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* '''Apr-20 Mo'''
{{Template:Science for Ukraine}}
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]  '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''


::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«
  2024 Week 18
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]


* '''Apr-29 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can I update DatLab 8 to the latest version?'''<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
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* '''Apr-30 Tu'''
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* '''May-01 We'''   
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* '''May-02 Th'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
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* '''May-03 Fr'''


:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
'''<big>Next Week</big>'''
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
2024 Week 19
 
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* '''Apr-21 Tu'''  
* '''May-06 Mo'''
[[File:CS.png|right|100px|Citrate synthase]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Apr-22 We'''  
* '''May-07 Tu'''  
 
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* '''Apr-23 Th'''  
* '''May-08 We'''  
 
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* '''Apr-24 Fr'''  
* '''May-09 Th'''  
[[File:ETC-.JPG|right|150px|Electron transport chain]]
:{|
:{|
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
|}
:from the O2k-Network »[[IT Padova Morosinotto T]]«
<br />
{| class="wikitable " border="1" style="width: 80%; height: 10em;"
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{{Template:Oroboros picture of the month}}
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* '''May-10 Fr'''

Revision as of 12:50, 2 May 2024

Gentle Science
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A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«
  • May-10 Fr
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