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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2024 Week 11


* '''Mar-11 Mo'''
2024 Week 18
 
* '''Apr-29 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
|'''How can I update DatLab 8 to the latest version?'''<br>
Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«  
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''Mar-12 Tu'''  
* '''Apr-30 Tu'''  
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* '''May-01 We'''   
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* '''Mar-13 We'''  
* '''May-02 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''''Rewarded'' Open Access - compensating authors'''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]
- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript
|}
|}
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* '''Mar-14 Th'''
* '''May-03 Fr'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
:from the O2k-Network »[[DE Leipzip Ost M]]«
|}
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* '''Mar-15 Fr'''  
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2024 Week 12
  2024 Week 19


* '''Mar-18 Mo'''
* '''May-06 Mo'''
::::: '''[[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' Mar 18-21
----
* '''Mar-19 Tu'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' What if ''Alert: Error in titrations!'' appears in your O2 analysis template file? '''<br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.<br>
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
- »'''[[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|O<sub>2</sub> flux analysis]]'''«<br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Mar-20 We'''  
* '''May-07 Tu'''  
 
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* '''Mar-21 Th'''  
* '''May-08 We'''  
 
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Do you handle well your Hamilton syringes? What is a cleaning cycle?'''<br>
"A syringe cleaning cycle consists of filling an empty syringe with a washing solution while immersing [...] also the base of the barrel into the washing solution, without fully removing the plunger, and emptying the syringe into the waste beaker". <br>
- »'''[[MiPNet19.14 SOP Hamilton microsyringes]]'''«<br>
- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
|}
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* '''Mar-22 Fr'''
* '''May-09 Th'''  
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* '''May-10 Fr'''

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr