Template:Current week ecosystem agenda: Difference between revisions

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
  2024 Week 05
 
* '''Jan-29 Mo'''
2024 Week 18
:::: '''»[[E-mit Winter School 2024 Brixen IT]]«''' Jan 28 - Feb 02
 
* '''Apr-29 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can I update DatLab 8 to the latest version?'''<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
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* '''Jan-30 Tu'''
* '''Apr-30 Tu'''  
:::: '''16:30  [[Gnaiger Erich|Erich Gnaiger]] presenting at [[E-mit Winter School 2024 Brixen IT]]''' - ''Coupling control and pathway control in mitochondrial high-resolution respirometry''
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* '''Jan-31 We'''  
* '''May-01 We'''  
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Can the S-pathway O<sub><small>2</small></sub> flux be derived by subtracting N-pathway O<sub><small>2</small></sub> flux from NS-pathway O<sub><small>2</small></sub> flux?'''<br>
No, the sum of the O<sub><small>2</small></sub> fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity: <br>
- »'''[[Additive effect of convergent electron flow]]'''« <br>
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
|}
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* '''Feb-01 Th'''  
* '''May-02 Th'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' ''“Doing the entire hands-on module from start to finish was the best for really “cementing” theory and hands-on application together.”'''''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
[[Cosgrove Richard]] from Eli Lilly Research Labs, on what he liked best on [[IOC162]]<br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- »'''[[O2k-Feedback]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''Feb-02 Fr'''  
* '''May-03 Fr'''
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2024 Week 06
  2024 Week 19


* '''Feb-05 Mo'''
* '''May-06 Mo'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]]
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
:from the O2k-Network »[[SK Bratislava Sumbalova Z]]«
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Feb-06 Tu'''
* '''May-07 Tu'''  
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* '''Feb-07 We'''  
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* '''Feb-08 Th'''  
* '''May-08 We'''  
 
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br>
"For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration."<br>
-»'''[[MiPNet09.12 O2k-Titrations]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
|}
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* '''Feb-09 Fr'''  
* '''May-09 Th'''  
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* '''May-10 Fr'''

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr
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