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  2020 Week 47
  2020 Week 48


* '''Nov-16 Mo'''  
* '''Nov-23 Mo'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
|'''Mitochondrial function is impaired in human iPSC-derived astrocytes infected with Zika virus.'''<br>
|'''Cell culture medium and dithionite titrations – a bad combination for an instrumental O<sub>2</sub> background test?'''<br>
:from the O2k-Network »[[BR Rio de Janeiro Galina A]]« and »[[BR Rio de Janeiro Institute Biomedical Chemistry]]«  
Cell culture media are not ideal for instrumental O<sub>2</sub> background test when using dithionite titrations because side reactions affecting the O<sub>2</sub> concentration and thus interfering with the test. Instead of cell culture media, use [[MiR05]], [[MiR06]] or a strongly buffered alkaline phosphate solution. <br>
*Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. [[Ledur 2020 Sci Rep|»Bioblast link«]]
-»[[MiPNet14.06 Instrumental O2 background]]« <br>
- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
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* '''Nov-17 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
* '''Nov-24 Tu'''
:::: [[ISCaM 2020 Virtual Event]]


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* '''Nov-18 We'''  
* '''Nov-25 We'''
 
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* '''Nov-19 Th'''
::::» <big>'''[[2020 PaduaMuscleDays Padua IT]]'''</big> / [[MiPNet25.04 IOC146 Padua IT|<big>'''IOC Padua IT'''</big>]]
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| '''What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?'''<br>
|'''Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.'''<br>
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.<br>
: from the O2k-Network »[[CA Guelph Holloway GP]]«
- »[[Permeabilized_muscle_fibers#Chemicals_and_media |Optimum ADP concentration in pfi]]« <br>
*Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. [[Politis-Barber 2020 Am J Physiol Endocrinol Metab|»Bioblast link«]]
- » [[Komlodi Timea]]« and » [[Doerrier Carolina]]«
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* '''Nov-20 Fr'''  
* '''Nov-26 Th'''  
:::: [[2020 PaduaMuscleDays Padua IT]]


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* '''Nov-27 Fr'''
:{|
:{|
| width="110" align="center" | [[File:O2k-Workshops.jpg|left|80px|O2k-Events|link=O2k-Events]]
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
| '''[[Doerrier Carolina|Dr. Carolina Doerrier]] and [[Gnaiger Erich|Dr. Erich Gnaiger]] will be presenting at the virtual [[2020 PaduaMuscleDays Padua IT]]'''<br>
|'''''HSP60'' knockdown and its effect on mitochondria’s role in pancreatic ductal adenocarcinoma''' <br>
:* 09:30 - Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers - »[[MiPNet25.04 IOC146 Padua IT|'''IOC Padua IT''']]«  <br>
 
:* 15:40 - Body mass excess, muscle mass, obesity and mitochondrial fitness
* Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161.  [[Zhou 2018 Cell Death Dis|»Bioblast link«]]
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* '''Nov-21 Sa'''
:::: [[2020 PaduaMuscleDays Padua IT]]

Revision as of 09:14, 23 November 2020

2020 Week 48
  • Nov-23 Mo
O2k-Open Support Take care Cell culture medium and dithionite titrations – a bad combination for an instrumental O2 background test?

Cell culture media are not ideal for instrumental O2 background test when using dithionite titrations because side reactions affecting the O2 concentration and thus interfering with the test. Instead of cell culture media, use MiR05, MiR06 or a strongly buffered alkaline phosphate solution.
MiPNet14.06 Instrumental O2 background« 
- communicated by »Komlodi Timea« and »Cecatto Cristiane« 


  • Nov-24 Tu
ISCaM 2020 Virtual Event

  • Nov-25 We
O2k-Publications in the MiPMap Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.
from the O2k-Network »CA Guelph Holloway GP«
  • Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. »Bioblast link«

  • Nov-26 Th

  • Nov-27 Fr
O2k-brief repository HSP60 knockdown and its effect on mitochondria’s role in pancreatic ductal adenocarcinoma
  • Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161. »Bioblast link«
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