Template:Current week ecosystem agenda: Difference between revisions

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  2020 Week 44
  2020 Week 45


* '''Oct-26 Mo'''  
* '''Nov-02 Mo'''  
:{|
:{|
| width="110" align="center" | [[File:TRANSMIT Acronym.jpg|left|70px|TRANSMIT|link=TRANSMIT]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|     '''"TRANSMIT: THE ROAD TO MEET THE MITOS"''' – the interactive [[TRANSMIT]] booklet for children is available for download in multiple languages <br>
|''' How can you measure mitochondrial membrane potential with O2k-Fluorometry? '''<br>
- »https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/ <br>
The extrinsic fluorophores safranin, TMRM or Rhodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations. <br>
- »'''[[Safranin]]'''«, »'''[[TMRM]]'''«, »'''[[Rhodamine 123]]'''« <br>
- communicated by »[[Komlodi Timea]]«, »[[Iglesias-Gonzalez Javier]]« and »[[Cecatto Cristiane]]«
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|}


:{|
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* '''Nov-03 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]


| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Did you know that oxygen flux can be converted to ''flux control ratios'' (''FCRs'') directly in DatLab?'''<br>
Use this function to easily plot your experimental traces as ''FCRs'', or to analyze your data.
<br>
- »'''[[Flux_control_ratio#DatLab|''FCR'' in DatLab]]'''« <br>
- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
|}
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* '''Oct-27 Tu'''
* '''Nov-04 We'''
:{|
| width="100" align="center" |[[Image:O2k-Network.png|left|70px|O2k-Network|link=O2k-Network]][[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
| '''The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab'''
:from the O2k-Network »[[CH Lausanne Place N]]« <br>
*Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. [[Burtscher 2020 iScience|»Bioblast link«]]
|}


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* '''Oct-28 We'''
:{|
:{|
| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' Flow or flux - what is the difference? '''<br>
|'''Six-month caloric restriction causes tissue-specific bioenergetic changes in mitochondria isolated from rat heart and skeletal muscle'''<br>
Rate can be expressed both as flow (''I'') and flux (''J''). Flow is used if the sample ''X'' can be quantified as [[count]] (''N<sub>X</sub>''; the number of cells, organisms), and changes proportionally with the size of ''X''. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.  <br>
:from the O2k-Network »[[BR Sao Paulo Kowaltowski AJ]]«
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalar  <br>
*Serna JDC, Caldeira da Silva CC, Kowaltowski AJ (2020) Functional changes induced by caloric restriction in cardiac and skeletal muscle mitochondria. J Bioenerg Biomembr 52:269-77.[[Serna 2020 J Bioenerg Biomembr|»Bioblast link«]]
- communicated by »[[Cecatto Cristiane]]« and »[[Komlodi Timea]]« and »[[Gnaiger Erich]]«
|}
|}
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* '''Oct-29 Th'''
* '''Nov-05 Th'''
:{|
 
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' Would you like to monitor O<sub>2</sub> flux in response to light?  '''<br>
Read about the PB-Module, the new module in the NextGen-O2k that will enable experiments in PhotoBiology! <br>
- »'''[[PB-Module]]'''«, »'''[[PhotoBiology]]'''«  <br>
- communicated by »[[Huete-Ortega Maria]]« and »[[Schmitt Sabine]]« and »[[Tindle-Solomon Lisa]]«
|}
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* '''Oct-30 Fr'''
* '''Nov-06 Fr'''
:{|
 
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
:::: Registration deadline for: [[Integrative Physiology of Exercise 2020 Virtual Event]]
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br>
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br>
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.  [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]
|}

Revision as of 09:17, 2 November 2020

2020 Week 45
  • Nov-02 Mo
MitoPedia
How can you measure mitochondrial membrane potential with O2k-Fluorometry?

The extrinsic fluorophores safranin, TMRM or Rhodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations.
- »Safranin«, »TMRM«, »Rhodamine 123« 
- communicated by »Komlodi Timea«, »Iglesias-Gonzalez Javier« and »Cecatto Cristiane«



  • Nov-04 We
O2k-Publications in the MiPMap Six-month caloric restriction causes tissue-specific bioenergetic changes in mitochondria isolated from rat heart and skeletal muscle
from the O2k-Network »BR Sao Paulo Kowaltowski AJ«
  • Serna JDC, Caldeira da Silva CC, Kowaltowski AJ (2020) Functional changes induced by caloric restriction in cardiac and skeletal muscle mitochondria. J Bioenerg Biomembr 52:269-77.»Bioblast link«

  • Nov-05 Th

  • Nov-06 Fr
Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event
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