Template:Current week ecosystem agenda

2020 Week 42

• Oct-12 Mo Targeting Metabesity 2020 Virtual Event Oct 12-15

If using the Amplex UltraRed assay, when should the background H2O2 calibration be performed? Background H2O2 calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H2O2 is prepared, or if a different respiration medium is used. As the H2O2 stock solution needs to be freshly prepared, it is recommended to perform a background H2O2 calibration before each experiment and at a minimum once per experimental day. - »How to find the background H2O2 calibration protocol in DatLab « select “AmR calibration” for the background H2O2 calibration protocol - communicated by »Komlodi Timea« and »Schmitt Sabine«

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• Oct-13 Tu

 »FAT4BRAIN School IOC147 Virtual Event«

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• Oct-14 We

Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? from the O2k-Network »US VA Richmond Lesnefsky EJ«  Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. »Bioblast link«

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• Oct-15 Th

How can you reduce the amount of biological sample needed for high-resolution respirometry? The O2k-sV-Module provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced. - »O2k-sV-Module«  - communicated by »Komlodi Timea« and »Schmitt Sabine«

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• Oct-16 Fr

Which SUIT protocol can you use to evaluate mitochondrial respiration of PBMC and platelets? The following SUIT protocols will guide you through the titration experiments for these cell models - »SUIT-001 O2 ce-pce D004« and »SUIT-002 O2 ce-pce D007a«  - communicated by »Cecatto Cristiane« and »Garcia-Souza Luiz F«

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• Oct-17 Sa

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• Oct-18 Su

»ISCaM 2020 Virtual Event«