Template:Current week ecosystem agenda: Difference between revisions
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* '''Oct-06 Tu''' - [ | * '''Oct-06 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]] | ||
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| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | ||
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|''' Background H<sub>2</sub>O<sub>2</sub> calibration: what is it and why perform it when using Amplex UltraRed assay?'''<br> | |''' Background H<sub>2</sub>O<sub>2</sub> calibration: what is it and why perform it when using Amplex UltraRed assay?'''<br> | ||
Background H<sub>2</sub>O<sub>2</sub> calibration refers to a set of H<sub>2</sub>O<sub>2</sub> titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay. <br> | Background H<sub>2</sub>O<sub>2</sub> calibration refers to a set of H<sub>2</sub>O<sub>2</sub> titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay. <br> | ||
- »'''[ | - »'''[[Amplex_UltraRed#Calibration_with_H2O2|Background H<sub>2</sub>O<sub>2</sub> calibration]]'''« <br> | ||
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« | - communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« | ||
|} | |} | ||
Revision as of 13:36, 5 October 2020
2020 Week 41
- Oct-05 Mo
- Oct-06 Tu - ÖGMBT Life Science Tuesdays
Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?
- from the O2k-Network »BR Sao Paulo Kowaltowski AJ«
- Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. »Bioblast link«
- Oct-07 We
Background H2O2 calibration: what is it and why perform it when using Amplex UltraRed assay?
Background H2O2 calibration refers to a set of H2O2 titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay.
- »Background H2O2 calibration«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
- Oct-09 Fr
