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Revision as of 08:08, 7 September 2020

2020 Week 37

Sep-07 Mo

Why it is necessary to perform multiple H₂O₂ calibrations when using the Amplex UltraRed (AmR) assay?

The sensitivity to H₂O₂ of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H₂O₂ calibration before sample addition, after sample addition and multiple times during the experiment.
- »Komlodi 2018 Methods Mol Biol«

The DLPs for AmR SUIT protocols already come with multiple steps of H₂O₂ calibrations implemented.
- » SUITbrowser«
- communicated by »Komlodi Timea« and »Cardoso Luiza«

Sep-08 Tu

Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung

from the O2k-Network »DE Giessen Weissmann N«, »FI Tampere Dufour E«, »FI Helsinki Jacobs HT« and »DE Jena Szibor M«

Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. »Bioblast link«

Sep-09 We

Sep-10 Th

What is the limitation of State 3 respiration compared to OXPHOS capacity?

OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P_i, fuel substrates and O₂. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of excess E-P capacity due to kinetic limitations of the phosphorylation system.
- »BEC: Mitochondrial physiology« - Table 3 and several figures.
- communicated by » Cecatto Cristiane « and » Komlodi Timea «

Sep-11 Fr

@@ Line 1: / Line 1: @@
-Week 36
+Week 37
+* '''Sep-07 Mo'''
-* '''Aug-31 Mo'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
+| '''Why it is necessary to perform multiple H<sub>2</sub>O<sub>2</sub> calibrations when using the [[Amplex UltraRed]] (AmR) assay?'''<br>
+The sensitivity to H<sub>2</sub>O<sub>2</sub> of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H<sub>2</sub>O<sub>2</sub> calibration before sample addition, after sample addition and multiple times during the experiment.  <br>
+- »[[Komlodi 2018 Methods Mol Biol]]« <br>
+The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H<sub>2</sub>O<sub>2</sub> calibrations implemented. <br>
+- » [https://www.bioblast.at/index.php/SUITbrowser SUITbrowser]« <br>
+- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
+|}
 ----
-* '''Sep-01 Tu'''
+* '''Sep-08 Tu'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction'''<br>
+|'''Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung'''<br>
-:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«
+:from the O2k-Network »[[DE Giessen Weissmann N]]«, »[[ FI Tampere Dufour E]]«, »[[FI Helsinki Jacobs HT]]« and »[[DE Jena Szibor M]]«
-*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]
+*Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. [[Sommer 2020 Sci Adv|»Bioblast link«]]
 |}
 ----
-* '''Sep-02 We'''
+* '''Sep-09 We'''
 ----
-* '''Sep-03 Th'''
+* '''Sep-10 Th'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br>
+OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P<sub>i</sub>, fuel substrates and O<sub>2</sub>. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of [[excess E-P capacity]] due to kinetic limitations of the phosphorylation system. <br>
+- »'''[[BEC_2020.1_doi10.26124bec2020-0001.v1|BEC: Mitochondrial physiology]]'''« - Table 3 and several figures. <br>
+- communicated by » [[Cecatto Cristiane]] « and » [[Komlodi Timea]] «
+|}
-----
-* '''Sep-04 Fr'''
-----
-* '''Sep-05 Sa'''
 ----
-* '''Sep-06 So'''
+* '''Sep-11 Fr'''
-::::* Abstract submission deadline - »[[ESSR 2020 Innsbruck AT]]«