Template:Current week ecosystem agenda: Difference between revisions
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- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« | - '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« | ||
- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid | - Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''« | ||
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Revision as of 21:37, 4 May 2020
- May-04 Mo
Do you load the correct instrumental background correction according to the actual range of experimental O2 concentrations?
Perform and apply the instrumental background test:
- » O2 background correction in DatLab« and » Instrumental oxygen background«
- May-05 Tu
Expression of alternative oxidase prevents reverse electron transfer
- - from the O2k-Network »FI Helsinki Jacobs HT« »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«
- Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - »Bioblast link«
- May-06 We
- May-07 Th
Quantify fatty acid oxidation capacity (FAO, F-pathway), avoiding a common artefact of overestimation. Malate anaplerotic pathways (see mt-malic enzyme) are active in various tissues and cultures cells, which requires caution: »Malate anaplerotic pathway control state« - DatLab 7.4 provides a step-by-step guide to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway capacity can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »SUIT-002«
- May-08 Fr
