Template:Current week ecosystem agenda: Difference between revisions

Revision as of 21:35, 4 May 2020

May-04 Mo

Do you load the correct instrumental background correction according to the actual range of experimental O₂ concentrations?

Perform and apply the instrumental background test:
- » O2 background correction in DatLab« and » Instrumental oxygen background«

May-05 Tu

Expression of alternative oxidase prevents reverse electron transfer

- from the O2k-Network »FI Helsinki Jacobs HT« »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«

Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - »Bioblast link«

May-06 We

May-07 Th

Quantify fatty acid oxidation capacity (FAO, F-pathway), avoiding a common artefact of overestimation. Malate anaplerotic pathways (see mt-malic enzyme) are active in various tissues and cultures cells, which requires caution: »Malate anaplerotic pathway control state«

- DatLab 7.4 provides a step-by-step guide to evaluate the malate anaplerotic pathway - »SUIT-027«

- Since F-pathway capacity can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid overestimation of FAO capacity - »SUIT-002«

May-08 Fr

@@ Line 2: / Line 2: @@
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
-|'''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?'''<br>
+|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br>
-See how to perform the instrumental background test.<br>
+Perform and apply the instrumental background test:<br>
 - »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
 |}
@@ Line 9: / Line 9: @@
 ----
 * '''May-05 Tu'''
-::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse'''
+::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''
 ::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«  »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«
-::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
+::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
 ----
 * '''May-06 We'''
@@ Line 20: / Line 20: @@
 :{|
 | width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
-|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
+| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate anaplerotic pathway control state]]'''«
-Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
-- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
+- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
-- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
-- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
+- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid overestimation of FAO capacity - »'''[[SUIT-002 |SUIT-002]]'''«
 |}
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