Template:Current week ecosystem agenda: Difference between revisions

Revision as of 08:24, 4 May 2020

May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

May-05 Tu

Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse

- from the O2k-Network »FI Helsinki Jacobs HT« »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«

Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. »Bioblast link«

May-06 We

May-07 Th

Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?

Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

May-08 Fr

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-* '''Apr-27 Mo'''
+* '''May-04 Mo'''
+:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?'''
+::::::: See how to perform the instrumental background test. - »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
-:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?'''
-::::::: To obtain an accurate O<sub>2</sub>  background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub>  levels. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«
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-* '''Apr-28 Tu'''
+* '''May-05 Tu'''
-::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''
+::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse'''
-::::::: - from the O2k-Network » [[FI Helsinki Jacobs HT]] « » [[DE Magdeburg Gellerich FN]] « and»[[DE Magdeburg Debska-Vielhaber G]]«
-::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. [[Szibor 2020 J Biol Chem|»Bioblast link«]]
+::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«  »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«
+::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
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+* '''May-06 We'''
-* '''Apr-29 We'''
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-* '''Apr-30 Th'''
+* '''May-07 Th'''
+:{|
+| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
+Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
+- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
+- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
+- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
+|}
+<br />
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-* '''May-01 Fr'''
+* '''May-08 Fr'''