Template:Current week ecosystem agenda: Difference between revisions

Revision as of 08:18, 27 April 2020

Apr-27 Mo

Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O₂ regime?

To obtain an accurate O₂ background correction, you must select the marks that correspond to your range of experimental O₂ levels. - » O2 Background correction - troubleshooting«

Apr-28 Tu

The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake

- from the O2k-Network » FI Helsinki Jacobs HT « » DE Magdeburg Gellerich FN « and»DE Magdeburg Debska-Vielhaber G«

Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. »Bioblast link«

Apr-29 We

Apr-30 Th

May-01 Fr

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-* '''Apr-20 Mo'''
+* '''Apr-27 Mo'''
-:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]  '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''
-::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«
+:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?'''
-::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523.  - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]
+::::::: To obtain an accurate O<sub>2</sub>  background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub>  levels. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«
+----
+* '''Apr-28 Tu'''
-:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
+::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]'''  The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake '''
-::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
+::::::: - from the O2k-Network » [[FI Helsinki Jacobs HT]] « » [[DE Magdeburg Gellerich FN]] « and»[[DE Magdeburg Debska-Vielhaber G]]«
+::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. [[Szibor 2020 J Biol Chem|»Bioblast link«]]
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-* '''Apr-21 Tu'''
+* '''Apr-29 We'''
-[[File:CS.png|right|100px|Citrate synthase]]
-:{|
-| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
-| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
-- »'''[[MiPNet17.04 CitrateSynthase]]'''«
-|}
-<br />
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-* '''Apr-22 We'''
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-* '''Apr-23 Th'''
+* '''Apr-30 Th'''
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-* '''Apr-24 Fr'''
+* '''May-01 Fr'''
-[[File:ETC-.JPG|right|150px|Electron transport chain]]
-:{|
-| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
-| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
-- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
-|}
-<br />