Template:Current week ecosystem agenda: Difference between revisions

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* '''Apr-13 Mo'''
* '''Apr-20 Mo'''  
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]  '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''
 
::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523.  - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]
 
 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«
 
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* '''Apr-21 Tu'''
[[File:CS.png|right|100px|Citrate synthase]]
:{|
:{|
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - »'''[[SUIT-024 |SUIT-024]]'''«
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
|}
|}
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* '''Apr-14 Tu'''  
* '''Apr-22 We'''  
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''
 
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«
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* '''Apr-23 Th'''


* '''Apr-15 We'''
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* '''Apr-24 Fr'''  
* '''Apr-16 Th'''
[[File:ETC-.JPG|right|150px|Electron transport chain]]
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''
:{|
 
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
::::::: - from the O2k-Network »[[DE Frankfurt Eckert GP]]« »[[CH Basel Eckert A]]«
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
::::::* Leuner K, Schulz K, Schütt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, Müller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204.  - [[Leuner 2012 Mol Neurobiol|»Bioblast link«]]
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
|}
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* '''Apr-17 Fr'''

Revision as of 09:00, 20 April 2020

  • Apr-20 Mo
O2k-brief repository Mitochondrial respiration medium MiR05 wins - KCl inhibits
- from the O2k-Network »US TN Nashville Wasserman DH« and »US MI East Lansing Bazil JN«
  • Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - »Bioblast link«


O2k-Open SupportInfo: improve Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - » Pyruvate - troubleshooting«

  • Apr-21 Tu
Citrate synthase
MitoPedia Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«



  • Apr-22 We

  • Apr-23 Th

  • Apr-24 Fr
Electron transport chain
MitoPedia ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«


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