Template:Chemicals SUIT-006 Q mt D071: Difference between revisions
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{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
{{Template:Chemicals header}} | {{Template:Chemicals header}} | ||
|- | |- | ||
| 1Rot | | 1Rot | ||
| {{Template:Rotenone}} | | {{Template:Rotenone}} | ||
| This step is essential to reach the fully oxidized | | This step is essential to reach the fully oxidized CoQ<sub>2</sub> needed to calculate the reduced Q fraction. | ||
|- | |- | ||
| 1S | | 1S | ||
Line 24: | Line 21: | ||
| 4U | | 4U | ||
| {{Template:CCCP}} | | {{Template:CCCP}} | ||
| Can be substituted for other [[MitoPedia:_Uncouplers|uncoupler]] | | Can be substituted for other [[MitoPedia:_Uncouplers|uncoupler]]. | ||
|- | |- | ||
| | | 5Anox | ||
| | | | ||
| | | The O<sub>2</sub> concentration in the O2k-chamber can be decreased by N<sub>2</sub> or H<sub>2</sub> injection to reach faster anoxia, see: [[Setting the oxygen concentration]]. | ||
|- | |- | ||
| | | 6Ama | ||
| {{Template:Antimycin A}} | | {{Template:Antimycin A}} | ||
| This step can be | | This step can be omitted. | ||
|} | |} |
Latest revision as of 16:23, 25 November 2021
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1Rot | Rotenone (Rot) | This step is essential to reach the fully oxidized CoQ2 needed to calculate the reduced Q fraction. |
1S | Succinate (S) | |
2D | ADP (D) | |
(3Omy) | Oligomycin (Omy) | This step can be skipped. |
4U | Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U) | Can be substituted for other uncoupler. |
5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
6Ama | Antimycin A (Ama) | This step can be omitted. |