Template:Chemical SUIT-034 NADH mt D082

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Step Chemical(s) and link(s) Comments
1D.1 ADP (D)
2PGM Pyruvate (P), Glutamate (G), and Malate (M)
3D2.5 ADP (D)
4U SF6847 We do not recommend the use of any other uncoupler, like Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to background fluorescence.
5Anox The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration.
6Myx Myxothiazol We do not recommend the use of any other inhibitor of complex III, like Antimycin A (Ama), due to the chemical background effect on fluorescence.
7Reox
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