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Difference between revisions of "Talk:SUIT-006 MgG mt D055"

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== Chemicals and syringes ==


{{Template:Chemicals SUIT-006 MgG mt D055}}
{{Template:Chemicals SUIT-006 MgG mt D055}}

Revision as of 17:26, 2 September 2020


Step Chemical(s) and link(s) Comments
1PM Pyruvate (P) and Malate (M) Can be substituted by other combination of substrates
2D ADP (D) preparation for MgG assay For this protocol, the ADP must be prepared without Mg2+ salts.
3Cat Carboxyatractyloside (Cat) Can be substituted for oligomycin
4U SF6847 Can be substituted for other uncoupler
5Ama Antimycin A (Ama) Can be substituted for other CIII or CIV inhibitor


Chemical and link Abbreviation Chemical and link
Magnesium Green MgG
MgCl2 Mg
Chemical and link Abbreviation Comments
Pyruvate P Can be substituted by other combination of substrates
Malate M Can be substituted by other combination of substrates
ADP D For this protocol, the ADP must be prepared without Mg2+ salts.
Carboxyatractyloside Cat Can be substituted for oligomycin
Uncoupler: in this DLP, SF6847 was used U Can be substituted for other uncoupler
Antimycin A Ama Can be substituted for other CIII or CIV inhibitor