Difference between revisions of "Talk:SUIT-006 MgG mt D055"
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== Chemicals and syringes | == Chemicals and syringes == | ||
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{{Template:Chemicals SUIT-006 MgG mt D055}} | |||
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! Chemical and link | ! Chemical and link | ||
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| [[Magnesium_Green#Preparation_of_MgG_solution|Magnesium Green]] | | [[Magnesium_Green#Preparation_of_MgG_solution|Magnesium Green]] |
Revision as of 17:21, 2 September 2020
Chemicals and syringes
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1PM | Pyruvate (P) and Malate (M) | Can be substituted by other combination of substrates |
2D | ADP (D) preparation for MgG assay | For this protocol, the ADP must be prepared without Mg2+ salts. |
3Cat | Carboxyatractyloside (Cat) | Can be substituted for oligomycin |
4U | SF6847 | Can be substituted for other uncoupler |
5Ama | Antimycin A (Ama) | Can be substituted for other CIII or CIV inhibitor |
Chemical and link | Abbreviation | Chemical and link |
---|---|---|
Magnesium Green | MgG | |
MgCl2 | Mg |
Chemical and link | Abbreviation | Comments |
---|---|---|
Pyruvate | P | Can be substituted by other combination of substrates |
Malate | M | Can be substituted by other combination of substrates |
ADP | D | For this protocol, the ADP must be prepared without Mg2+ salts. |
Carboxyatractyloside | Cat | Can be substituted for oligomycin |
Uncoupler: in this DLP, SF6847 was used | U | Can be substituted for other uncoupler |
Antimycin A | Ama | Can be substituted for other CIII or CIV inhibitor |