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Talk:Permeabilization of plasma membrane

From Bioblast


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Talk:Permeabilization of plasma membrane

Description

Permeabilization of plasma membrane with optimal digitonin or saponin concentrations yields an effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes. Since the plasma membrane is impermeable to ADP, cytochrome c, and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.


Several compounds (e.g. ADP, cytochrome c,) are not cell membrane-permeable, therefore OXPHOS analysis requires isolation of mitochondria or selective plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes making externally added compounds accessible to the mitochondria. Cell membrane can be permeabilized mechanically (e.g. liver tissue) and by mild detergents (i.e. digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.


Reference: Doerrier 2018 Methods Mol Biol








MitoPedia topics: Sample preparation, Mitochondrial preparations