Difference between revisions of "Talk:Permeabilization of plasma membrane"
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|description= Selective '''permeabilization of plasma membrane''' or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (''e.g.,'' [[ADP]], [[Cytochrome c|cytochrome ''c'']]) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (''e.g.,'' liver tissue) and by mild detergents (''i.e.,'' [[digitonin]] or [[saponin]]). See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]]. | |description= Selective '''permeabilization of plasma membrane''' or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (''e.g.,'' [[ADP]], [[Cytochrome c|cytochrome ''c'']]) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (''e.g.,'' liver tissue) and chemically by mild detergents (''i.e.,'' [[digitonin]] or [[saponin]]). See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]]. | ||
|info=[[Doerrier 2018 Methods Mol Biol]] | |info=[[Doerrier 2018 Methods Mol Biol]] | ||
Revision as of 13:46, 20 July 2020
- high-resolution terminology - matching measurements at high-resolution
Talk:Permeabilization of plasma membrane
Description
Selective permeabilization of plasma membrane or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (e.g., ADP, cytochrome c) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (e.g., liver tissue) and chemically by mild detergents (i.e., digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia methods: Respirometry
MitoPedia topics: Sample preparation
- » MitoPedia topic: Mitochondrial preparations
