Difference between revisions of "Talk:Permeabilization of plasma membrane"
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|description='''Permeabilization of plasma membrane''' with optimal [[digitonin]] or [[saponin]] concentrations yields an effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes. Since the plasma membrane is impermeable to [[ADP]], [[Cytochrome c]], and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]]. | |description='''Permeabilization of plasma membrane''' with optimal [[digitonin]] or [[saponin]] concentrations yields an effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes. Since the plasma membrane is impermeable to [[ADP]], [[Cytochrome c|cytochrome ''c'']], and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]]. | ||
Several compounds (e.g. [[ADP]], [[Cytochrome c|cytochrome ''c'']],) are not cell membrane-permeable, therefore OXPHOS analysis requires isolation of mitochondria or selective '''plasma membrane permeabilization''' with effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes making externally added compounds accessible to the mitochondria. Cell membrane can be permeabilized mechanically (e.g. liver tissue) and by mild detergents (i.e. [[digitonin]] or [[saponin]]). See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]]. | |||
|info=[[Doerrier 2018 Methods Mol Biol]] | |info=[[Doerrier 2018 Methods Mol Biol]] | ||
}} | }} | ||
Revision as of 15:52, 16 July 2020
- high-resolution terminology - matching measurements at high-resolution
Talk:Permeabilization of plasma membrane
Description
Permeabilization of plasma membrane with optimal digitonin or saponin concentrations yields an effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes. Since the plasma membrane is impermeable to ADP, cytochrome c, and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.
Several compounds (e.g. ADP, cytochrome c,) are not cell membrane-permeable, therefore OXPHOS analysis requires isolation of mitochondria or selective plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes making externally added compounds accessible to the mitochondria. Cell membrane can be permeabilized mechanically (e.g. liver tissue) and by mild detergents (i.e. digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia topics:
Sample preparation,
Mitochondrial preparations
