Difference between revisions of "Talk:Permeabilization of plasma membrane"

Latest revision as of 17:46, 21 July 2020

high-resolution terminology - matching measurements at high-resolution

Talk:Permeabilization of plasma membrane

Description

Selective permeabilization of plasma membrane or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (e.g., ADP, cytochrome c) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (e.g., liver tissue) and chemically by mild detergents (i.e., digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue, Permeabilized tissue or cells.

» MitoPedia topic: Mitochondrial preparations

Reference: Doerrier 2018 Methods Mol Biol

MitoPedia methods: Respirometry

MitoPedia topics: Sample preparation

MitoPedia: permeabilization of plasma membrane

References

Bioblast link	Reference	Year
BEC 2020.1 doi10.26124bec2020-0001.v1	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1	2020

Labels:

Preparation: Permeabilized tissue, Permeabilized cells, Permeabilized muscle fibers

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 {{MitoPedia
-|description='''Permeabilization of plasma membrane''' with optimal [[digitonin]] or [[saponin]] concentrations  yields an effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes. Since the plasma membrane is impermeable to [[ADP]], [[Cytochrome c|cytochrome ''c'']], and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]].
+|description= Selective '''permeabilization of plasma membrane''' or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (''e.g.,'' [[ADP]], [[Cytochrome c|cytochrome ''c'']]) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (''e.g.,'' liver tissue) and chemically by mild detergents (''i.e.,'' [[digitonin]] or [[saponin]]). See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]], [[Permeabilized tissue or cells]].
-Several compounds (''e.g.'' [[ADP]], [[Cytochrome c|cytochrome ''c'']],) are not cell membrane-permeable, therefore OXPHOS analysis requires isolation of mitochondria or selective '''plasma membrane permeabilization''' with effective wash-out of free cytosolic molecules including [[adenylates]], [https://wiki.oroboros.at/index.php/MitoPedia:_Substrates_and_metabolites substrates], and cytosolic enzymes making externally added compounds accessible to the mitochondria. Cell membrane can be permeabilized mechanically (''e.g.'' liver tissue) and by mild detergents (''i.e.'' [[digitonin]] or [[saponin]]). See also: [[Permeabilized muscle fibers]], [[Permeabilized cells]], [[Permeabilized tissue]].
+:» MitoPedia topic: [[Mitochondrial preparations]]
 |info=[[Doerrier 2018 Methods Mol Biol]]
 }}
 {{MitoPedia concepts}}
-{{MitoPedia methods}}
+{{MitoPedia methods|mitopedia method=Respirometry}}
-{{MitoPedia O2k and high-resolution respirometry
+{{MitoPedia O2k and high-resolution respirometry}}
-|mitopedia  MiP and biochemistry =sample preparations}}
+{{MitoPedia topics
+|mitopedia topic=Sample preparation}}
 __TOC__
+== MitoPedia: permeabilization of plasma membrane ==
-{{MitoPedia topics
+::::* [[Permeabilized tissue]], pti
-|mitopedia topic=Sample preparation,
+::::* [[Permeabilized muscle fibers]], pfi
-[[Mitochondrial preparations]]}}
+::::* [[Permeabilized cells]], pce
+== References ==
+{{#ask:[[Additional label::Permeabilized muscle fibers]]
+| mainlabel=Bioblast link
+|?Has title=Reference
+|?Was published in year=Year
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Has title
+|order=ascending
+}}
+{{Labeling
+|preparations=Permeabilized tissue, Permeabilized cells, Permeabilized muscle fibers }}