Talk:MitoCom O2k-Fluorometer
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MitoCom O2k-Fluorometer Report 2011-2014
Dissemination
Public awareness
Year 3:
- eco.nova tirol innovativ - November 2014
- Tiroler Wirtschaft - August 2014
- Weekend Magazin Tirol - July 2014
- tiroler LANDESZEITUNG - May 2014
- TWInside - April 2014
- Branchenkatalog Mitocom Tyrol, OROBOROS INSTRUMENTS WGT Elektronik GmbH & Co KG - July 2013
- MUI Thema July 3rd
- Forum MedizinUni - June 20th, 2013
- mypoint MUI - June 20th, 2013
- Tiroler Tageszeitung - May 29th, 2013
Year 2:
- Standort 04/2012 2012
- zukunft forschung 2012
- K-Regio Folder 2012
- Forum MedizinUni, Beilage der Tiroler Tageszeitung - June 14th, 2012
- Freizeit Kurier-Artikel: Kraftwerk - June 2nd, 2012
- EURAC Bozen - June 1st, 2012
- Standard-Artikel:Neues Licht auf das Körperkraftwerk - May 16th, 2012
Year 1:
- Houska Preis 2011 - Siegerprojekt:
- Präsentation Science2Business
- Vortrag Berlin
- K-Regio beim Land Tirol
- K-Regio Standort Artikel I
- Die_Kraftwerke_der_Zellen Standort Artikel II
Overview on internet presentations of K-Regio MitoCom
- MitoCom Network
- OROBOROS INSTRUMENTS: http://www.oroboros.at/index.php?mitocom-tyrol
MitoCom Workshops
- O2k-Workshop on HRR and O2k-Fluorometry, 2014 October 07-12, Schroecken, Tryol (AT)
- O2k-Fluorometry Workshop (advanced), 2014 September 12-16, Obergurgl, Tyrol (AT)
- O2k-Basic Workshop 2014 April 07-12, Schroecken, Voralberg (AT)
- O2k-Basic Workshop2013 Oct 9-14, Schroecken, Voralberg (AT)
- O2k-Fluorometry Workshop2013 Sept 19-23, Obergurgl, Tyrol (AT)
- intern: MitoCom Workshop May 16
- O2k-Workshop IOC66 O2k-Fluorescence and High-Resolution Respirometry. 2012 March 15 to 16; Innsbruck, Austria. - Feedback
Publications K-Regio MitoCom
International peer-reviewed journals
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2014) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol [in press].
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181.
- Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7(4): e34890.
- Kristiansen G, Hu J, Wichmann D, Stiehl DP, Rose M, Gerhardt J, Bohnert A, ten Haaf A, Moch H, Raleigh J, Varia MA, Subarsky P, Scandurra FM, Gnaiger E, Gleixner E, Bicker A, Gassmann M, Hankeln T, Dahl E, Gorr TA (2012) Endogenous myoglobin in breast cancer is hypoxia-inducible by alternate transcription and functions to impair mitochondrial activity: a role in tumor suppression? J Biol Chem 286: 43417-43428
- Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.
- Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
MiPNet Publications
- MiPNet19.20 Amplex Red Data Acquisition and Analysis
- MiPNet19.19 Safranin Data Acquisition and Analysis
- MiPNet18.06 Amplex-Yeast
- MiPNet18.05 Amplex-Mouse-heart
- MiPNet17.18._Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. ISBN: 978-3-9502399-6-6
- MiPNet17.17 Amplex-Mouse-brain
- MiPNet17.16 O2k-Spectrophotometry
- MiPNet17.12_Bioblast 2012: Mitochondrial Competence. ISBN 978-3-9502399-5-9
- MiPNet17.06_IOC66_2012-03_Innsbruck
- G MiPNet17.05 O2k-Fluorescence LED2-Module
- MiPNet17.04_CitrateSynthase
- MiPNet17.03_Shredder_vs_Fibres
- MiPNet17.02_PBI-Shredder-Manual
Abstracts: Invited lectures at international conferences
- Gnaiger E, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet J, Christiansen M, Helge JW, Saltin B, Boushel R (2013) Conserved Mitochondrial Coupling Efficiency and Adaptive Substrate Control in Inuit and Caucasians: Adaptation and Acclimatization to the Arctic Winter. Abstract Mito2013, Tokyo, JP. November 6-7
- Laner V, Gradl P, Eigentler A, Fontana-Ayoub M, Fasching M, Gnaiger E (2013) The OROBOROS Oxygraph-2k for High-Resolution Respirometry and O2k-Fluorometry: A New Quality in OXPHOS Analysis. Luncheon Seminar Abstract ASMRM 2013, Seoul, KR. November 4-5.
- Gnaiger E (2013)Loss of mitochondrial competence and degenerative disease: cause and effect. Abstract ASMRM 2013, Seoul, KR. November 4-5.
- Gnaiger E (2013) Biochemical coupling efficiency in permeabilized fibres from arm and leg muscle in Inuit versus Caucasians: A functional test of the uncoupling hypothesis in Greenland. Abstract MiP2013, Obergurgl, Austria. September 23-27.
- Gnaiger E (2013) Body mass index and the risk of neurodegeneration: from mitochondrial fitness to brain function. Abstract Neurocon 2013, Santiago Kolkata, India. Symposium: Neurocon 2013. January 17-20.
- Gnaiger E (2012) Aging and mitochondrial physiology. The effect of exercise and caloric balance on oxidative phosphorylation (OXPHOS). Abstract FEPS, Santiage de Compostela, Spain. Symposium: Advances in Aging and Mitochondria Research. September 08-12.
- Gnaiger E, Wright-Paradis C, Sondergaard H, Calbet J, Damsgaard R, Munck-Andersen T, Saltin B, Boushel R (2011) Conserved Mitochondrial Coupling and Adaptive Substrate Control in Inuit and Caucasians: Acclimatization to Active Life Styles in the Arctic Winter. Abstract SMRM, Hyderabad, India.
- Gnaiger E (2011) Fewer mitochondria - mitochondrial 'fever': Mitochondrial physiology and capacity of oxidative phosphorylation in active and sedentary life styles. Abstract Targeting Mitochondria, Berlin, DE.
- Sumbalova Z, Wiethuechter A, Fasching M, Gnaiger E (2011) Coupling control and substrate control of mitochondrial membrane potential and respiration in the mouse brain, and comparison with skeletal and cardiac muscle. Abstract MiP2011 Bordeaux, FR.
- Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima, Japan.
Abstracts: Poster presentations at international conferences
- Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism. Abstract MiP2011 Bordeaux, FR.
- Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract MiP2011, Bordeaux, FR; and Targeting Mitochondria, Berlin, DE.
- Pesta D, Wiethuechter A, Karall D, Schocke M, Gnaiger E (2011) Functional mitochondrial diagnosis in a patient suffering from sudden exercise intolerance. Abstract Mitochondrial Medicine Chicago.
- Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Mitochondrial improvements after strength, endurance, and intermittend hypoxic training in sedentary humans. A respiratory study on permeabilized muscle fibres. Abstract Mitochondrial Medicine Chicago, USA.
- Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane potential. Abstract Mitochondrial Medicine Chicago.
- Burtscher M, Dietrich H, Gatterer H, Gnaiger E, Harrison DK, Karall D, Krismer M, Lass-Floerl C, Parson W, Perkhofer S, Pesta D, Schocke M, Sumbalova Z, Wiethüchter A (2011) Mitochondrial competence. Regional and international cooperation for establishing new standards in functional mitochondrial diagnosis. Abstract Mitochondrial Medicine Chicago, USA.
Exhibitions at international conferences and workshops
- 2014-07-12 to 15: EBEC2014, Lisbon, PT Erich Gnaiger
- 2014-06-04 to 07: EUROMIT2014, Tampere, FI: Erich Gnaiger, Verena Laner
- 2014-05-15 to 19: UMDF2014, Pittsburgh, US: Erich Gnaiger, Verena Laner
- 2014-05-25 to 31: World Congress on High Altitude Medicine and Physiology (ISMM2014), Bolzano, IT: Erich Gnaiger, Verena Laner
- 2014-01-27 to 30: ]http://www.bioblast.at/images/f/f2/MiPNet18.14_IOC85_Mahabaleshwar.pdf 38th Mahabaleshwar Seminar, Mahabaleshwar, IN]: Erich Gnaiger
- 2013-12-19 to 21: SMRM2013, Bangalore, India: Erich Gnaiger
- 2013-11-06 to 07: Mitochondria2013, Tokyo, JP: Erich Gnaiger, Verena Laner
- 2013-11-04 to 05: ASMRM, Seoul, KR: Invited lectured and luncheon seminar Erich Gnaiger.
- 2013-09-23 to 27: MiP2013Obergurl, Austria. Erich Gnaiger, abstract presentation
- 2013-04-09 to 15: IOC75: Tutoring Mona Fontana-Ayoub
- 2013-02-18: Clinical Hypothermia and Mitochondrial Function Verena Laner, Erich Gnaiger
- 2013-01-17 to 20: Neurocon 2013, Kolkata, IN: Verena Laner, Erich Gnaiger
- 2013-01-14 to 16: Pre-conference O2k-Workshop Neurocon 2013, Kolkata, IN: Verena Laner, Erich Gnaiger
- 2012-12-05 to 10: IOC72 Tutoring Mona Fontana-Ayoub
- 2012-09-15 to 20: EBEC 2012, Freiburg i. Breisgau, DE: Katrin Stecher, Verena Laner, Erich Gnaiger
- 2012-05-07: O2k-Fluorometry Mitolab Workshop Madrid: Erich Gnaiger
- 2012-03-15 to 16: IOC66 - O2k-Fluorescence. An International O2k-Workshop of MitoCom Tyrol.
- 2011-10-20 to 21: Targeting Mitochondria, Berlin, DE. Exhibition of OROBOROS INSTRUMENTS and MitoCom Tyrol: Katrin Stecher, Lydia Staudacher.
- 2011-09-05 to 04: MiP2011 Bordeaux, France. Exhibition of OROBOROS INSTRUMENTS and MitoCom Tyrol: Katrin Stecher, Lydia Staudacher.
- 2011-08-31 to 09-04: Kagoshima, Japan. Exhibition of the OROBOROS Oxygraph-2k. Yoshiaki Nakayoshi, Sanyo Trading Co. Ltd., Tokyo, JP and Erich Gnaiger, OROBOROS INSTRUMENTS, AT.
- 2011-06-15 to 18: UMDF Mitochondrial Medicine 2011 - Chicago, US. Exhibition of OROBOROS INSTRUMENTS and MitoCom Tyrol. Katrin Stecher, Dominik Pesta, Erich Gnaiger.
Exhibitions and presentations at regional and national events
- 2012-11-06: MCI Forum Biotechnologie
- 2012-09-27: Tiroler Standort Lunch in Wien
- 2012-09-20: Tiroler Medizintechnikforum
International cooperations
Semmelweiss Univ. Budapest, HU
Univ. Granada, ES
Zewail City for Science and Technology, ET
University of Cambdrige, UK
Management
- 2011-10-30: ORO installed an 'Intern wiki' for communication, documentation and organization of the K-Regio Project MitoCom.
MitoCom Track Record 2012
O2k-Workshop, Schroecken, Austria, Dec 5 - 10
Bioblast 2012 - Conference on Mitochondrial Competence
The Bioblast Conference 2012 took place in Hotel Grauer Bär from 10th to 12th December 2012. With more than 70 participants from 19 nations the conference was a great success.
For the article of the Medical University Innsbruck, see: MUI website
More information: Bioblast 2012
Padua Muscle Days, Nov 23 - 25
After the satellite meeting in Vienna on October 17th, Prof. Dr. Gnaiger was invited by Prof. Dr. Ugo Carraro (University of Padua) to Padua for a talk on high-resolution respirometry in permeabilized fibers from small biopsies of human muscle.
MCI Forum Technologie & Life Sciences, Innsbruck, Nov 6
In her talk, Mag. Verena Laner will outline the development of the company OROBOROS INSTRUMENTS and will walk you through the early company days up to the current K-Regio Project MitoCom Tyrol. Programme
Satellite Meeting Vienna, Oct 17
Dr. Erich Gnaiger participated at the Satellite Meeting in Vienna, which took place on 17th October.
Attendees of this meeting were Delegates of the University in Padua (Univ.-Prof. Dr. Ugo Carraro and Dr. Sandra Zampieri), University in Chieti (Univ.-Prof. Dr. Protasi) and University in Rome (Univ.-Prof. Dr. Andrea Musaro).
The meeting was organized by Dr. Kern from the Ludwig Boltzmann Institute in Vienna.
Standort Lunch Vienna, 2012 Sep 27
Dr. Erich Gnaiger presented the MitoCom project at the Standort Lunch in Vienna, organized by the Standortagentur Tirol. More information.
The article of the STANDORT Tirol you can find in issue no. 15, page 8
Medizintechnikforum Hall, 2012 Sep 20 to 21
OROBOROS INSTRUMENTS exhibited at the Medizintechnikforum Hall, showing latest developments in high-resolution respirometry in combination with the O2k-Fluorescence LED2-Module. MitoCom Tyrol was presented together with the Open Innovation project, both supported by the Government of Tyrol.
Tiroltag, Europäisches Forum Alpbach, 2012 Aug 19
Verena Laner, the project manager of MitoCom Tyrol, took part at the Tirolday, which was the "Opening" of the European Congress in Alpbach 2012. The main topic was "The future of sports in the Alps", which is an integral part of the MitoCom project. Additionally to the presentations and the award presentation ceremony for young scientists, there was a podium discussion with well-known experts such as the famous alpinist Reinhard Messner.
MitoCom Cooperation AltitudeOmics 2012, 2012 July 20 to Aug 13
Dr. Erich Gnaiger participated in the AltitudeOmics2012 project in Bolivia and provided the scientists with two O2ks and his expertise. Aim of the study was to investigate the impacts of hypoxia/acclimatization onto molecular mechanisms of the human cells.
Visiting scientist: Prof. Dr. Fr. Baby Joseph, July 19
On July 19th, Prof. Dr. Fr. Baby Joseph visited the OROBOROS office.
Time schedule of the research visit
Houska Award 2011 - First price for MitoCom Tyrol, April 26
- First price - Medical University of Innsbruck: http://www.oroboros.at/?Houska-Award_2011
- http://www.houskapreis.at/siegerprojekt-2011-licht-in-die-kraftwerke-der-zellen
- http://www.houskapreis.at
- http://www.tt.com/Nachrichten/4702341-6/houska-preise-an-universit%C3%A4ten-in-innsbruck-und-graz.csp
Mitolab O2k-Fluorometry Workshop Madrid, Spain, May 7
- Mitolab Workshop by Erich Gnaiger
- Contact: Eduardo Rial (Email: [email protected]) and Susana Cadenas (Email: [email protected])
>> See also MitoCom_Tyrol - Report
O2k-Workshop Schröcken, Austria, April 11 to 16
O2k-Workshop Sydney, Australia, April 2 to 4
First O2k-Fluorometry Workshop, Innsbruck, March 15 to 16
- IOC66 O2k-Fluorometry Workshop. An International O2k-Workshop of MitoCom Tyrol.
Manuscript accepted for publication in PloS One, March 9
- "Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle" (by Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D) was accepted for publication in PloS One.
- From the anonymous referee's comments:
- Reviewer #1: "This is an original work performed with state of the art methodologies, as high-resolution respirometry. The results indicate that not only mitochondrial capacity is a limiting factor of horse performance but also the modalities of energy substrate utilization. Horse is an animal of choice for energetic studies since it shows a huge consumption of oxygen during muscle contraction, suggesting an positive adaptation of the mitochondrial system as compared to other animals. .. This article provides new findings on the link between mitochondrial capacity and whole animal body size and aerobic capacity. It also clarifies whether mitochondrial capacity is a controling factor for horse performance. An important finding is also that the control by the phosphorylation system is stable and does not vary with the performance of the horse. In contrast, the relative utilization of complex I versus complex II varies witht he performance, and this is a new finding which adds up to our knowledge of energy regulation. This work is of broad interest for sport physiology, metabolic researchers and aging research."
- Reviewer #2: "The results could be described in a much easier way if utilizing classical definitions of mitochondrial respiratory parameters (from the late Britton Chance's milestones), such as state IV (or II), state III, uncoupled respiration, respiratory control ratio and so on." (Comment: MitoPedia: Respiratory states)
