Difference between revisions of "Talk:Measuring hydrogen peroxide"
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=== Nicolas Pichaud === | === Nicolas Pichaud === | ||
We are presently doing a test with a marker called CellRox from Molecular Probes but you need a confocal microscope. It is working pretty well so far, we just need to do a bit more of optimization... I'll send you the protocol when it is ready. | We are presently doing a test with a marker called CellRox from Molecular Probes but you need a confocal microscope. It is working pretty well so far, we just need to do a bit more of optimization... I'll send you the protocol when it is ready. | ||
=== Daniel Munro === | |||
Permeabilized fibres still contain most of their antioxidants. I wonder to what extent they compete with Amplex Red for the H2O2. The results will certainly not represent actual rate of mitochondrial ROS production but rather a combination of ROS production and the strength of the antioxidant defences. | |||
Revision as of 15:46, 25 October 2013
MiPNet discussion forum: hydrogen peroxide production in intact muscle fibers (2013-10-25)
Marcus Olivera
I´ve been working on intact insect flight muscle to evaluate respiratory capacities but I´m experiencing many problems in detecting changes in rates of Amplex red fluorescence on physically permeabilized flight muscle. Does anyone know a suitable method to detect hydrogen peroxide generation on intact muscle fibers (not isolated mitochondria)?
Nicolas Pichaud
We are presently doing a test with a marker called CellRox from Molecular Probes but you need a confocal microscope. It is working pretty well so far, we just need to do a bit more of optimization... I'll send you the protocol when it is ready.
Daniel Munro
Permeabilized fibres still contain most of their antioxidants. I wonder to what extent they compete with Amplex Red for the H2O2. The results will certainly not represent actual rate of mitochondrial ROS production but rather a combination of ROS production and the strength of the antioxidant defences.
