Talk:Fluorescence-Sensor: Difference between revisions

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== Different sensitivities of sensors ==


Why are there differences in sensitivities between individual sensors?
Differences in sensitivity between sensors may be caused by    different luminous intensities of LEDs, slightly different    geometrical alignments of the LED in respect to measuring chamber    and photodiode, different photo sensitivities of photodiodes, and    different absorption characteristics of filters.  We observed strong    effects on the observed signal when the orientation (rotation,    angle) of an LED was slightly changed. Further, we measured the    light intensities recoded by the photodiode of each sensor (n = 16)    using either the sensors integrated LED as light source (experiment    A) and using an external (always the same) LED as light source    (experiment B). Although the geometric reproducibility of experiment    B was problematic, the standard deviation was reduced from 7% in    experiment A to 2% in experiment B. This indicates that the    variation of LED luminosity and small variations in LED alignment    may be the main contributing factors to the total variance in sensor    sensitivity. In fact, the variance of LED luminosity is a well known    problem, partially addressed by LED manufacturers in a pre-sorting    process ("binning" or "ranking"). Even within such pre-sorted "bins"    (that are not always available) manufacturers state typical    luminosity variations of +/-15 to +/- 20%, if any statement is given    at all.
Different sensitivities do not have adverse effects after calibration.

Revision as of 16:40, 15 May 2014

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