Difference between revisions of "Talk:Cytochrome c"
From Bioblast
| Line 12: | Line 12: | ||
:::: [[Cytochrome_c#Application_in_HRR:_storage_and_stock_solution_of_c|Cytochrome ''c'']] (from equine heart) from Sigma Aldrich used in high-resolution respirometry to test the integrity of the mitochondrial outer membrane is currently out of stock for a few months. Does anyone have experience using cytochrome ''c'' from another source (e.g. from bovine heart)? | :::: [[Cytochrome_c#Application_in_HRR:_storage_and_stock_solution_of_c|Cytochrome ''c'']] (from equine heart) from Sigma Aldrich used in high-resolution respirometry to test the integrity of the mitochondrial outer membrane is currently out of stock for a few months. Does anyone have experience using cytochrome ''c'' from another source (e.g. from bovine heart)? | ||
=== Carolina Doerrier, Oroboros | === [[Doerrier Carolina|Carolina Doerrier]], [[AT Innsbruck Oroboros]] === | ||
:::: For high-resolution respirometry, we only have experience with the cytochrome ''c'' listed on the Bioblast page. For different reasons, we think that it should not be any problem to use cytochrome ''c'' from bovine heart. First, cytochrome ''c'' is a highly conserved protein across different species, and second, Sigma Aldrich provides the same specifications for C7752 (cytochrome ''c'' from equine heart) and C2037 (cytochrome ''c'' from bovine heart). | :::: For high-resolution respirometry, we only have experience with the cytochrome ''c'' listed on the Bioblast page. For different reasons, we think that it should not be any problem to use cytochrome ''c'' from bovine heart. First, cytochrome ''c'' is a highly conserved protein across different species, and second, Sigma Aldrich provides the same specifications for C7752 (cytochrome ''c'' from equine heart) and C2037 (cytochrome ''c'' from bovine heart). | ||
=== [[Van den Bergh Francoise|Francoise Van den Bergh]], [[US MI Ann Arbor Beard DA]] === | |||
:::: Our lab uses cytochrome ''c'' from bovine heart (Sigma Aldrich: C2037). For the preparation of 4 mM cytochrome ''c'', we follow the information from [[Cytochrome_c#Application_in_HRR:_storage_and_stock_solution_of_c|MitoPedia]]. We buy the 50 mg bottle, so we do not need to weight it, we just add 1 mL H<sub>2</sub>O to the whole bottle, and then we aliquot it. | |||
Revision as of 09:27, 19 July 2021
Popular Bioblast page
- Cytochrome c has been accessed more than
- 15,000 times (2019-12-12)
- 10,000 times (2016-06-02)
- 5,000 times (2014-06-11)
- Cytochrome c has been accessed more than
MiPNet discussion forum: cytochrome c source (2021-07-16)
Question (US CA San Diego Patel HH)
- Cytochrome c (from equine heart) from Sigma Aldrich used in high-resolution respirometry to test the integrity of the mitochondrial outer membrane is currently out of stock for a few months. Does anyone have experience using cytochrome c from another source (e.g. from bovine heart)?
Carolina Doerrier, AT Innsbruck Oroboros
- For high-resolution respirometry, we only have experience with the cytochrome c listed on the Bioblast page. For different reasons, we think that it should not be any problem to use cytochrome c from bovine heart. First, cytochrome c is a highly conserved protein across different species, and second, Sigma Aldrich provides the same specifications for C7752 (cytochrome c from equine heart) and C2037 (cytochrome c from bovine heart).
Francoise Van den Bergh, US MI Ann Arbor Beard DA
- Our lab uses cytochrome c from bovine heart (Sigma Aldrich: C2037). For the preparation of 4 mM cytochrome c, we follow the information from MitoPedia. We buy the 50 mg bottle, so we do not need to weight it, we just add 1 mL H2O to the whole bottle, and then we aliquot it.
