Talk:Bioblast alert 2015

Bioblast alert - in preparation

Bioblast alert 2013(02): 2013-08-08

• MitoPedia news: CCCP - Carbonyl cyanide m-chloro phenyl hydrazone: In our on-going experiments with various sources of mitochondria and different mt-preparations, we observed that results on respiration and mt-membrane potential were fully comparable using CCCP or FCCP as uncouplers in parallel experiments. Therefore, from now on our lab will replace FCCP by CCCP using slightly higher optimum concentrations for the estimation of ETS capacity. Importantly, CCCP is far less expensive than FCCP (protocol development supported by MitoCom).
• Selection of appropriate methodologies: A recently published review by Perry and colleagues provides a critical assessment of currently used methodologies and instruments used in respirometry – excellent reading. Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.
• MitoPedia Glossary - new section: Respiratory control ratios.

Topics not yet covered

• MiPMap and comparative mitochondrial physiology
• MitoGlobal: Consortia and research centres with a focus on mitochondrial research and medicine are listed as MitoGlobal_Centres.
• Topic: O2-kinetics
• Preparation: Homogenate/isolated mitochondria; intact/permeabilized cells; permeabilized fibre/in vivo
• Protocols

Bioblast alert - concept

Here is a brief summary on the concept of Bioblast alert. Some of the following topics (not more than four per alert) will be selected for a Bioblast alert.

High-resolution terminology

• high-resolution terminology - matching measurement at high-resolution. Term of the week: Basal respiration
• Define – Measure: terminology and experimental approach
• Define – Clarify: consider discrepancies, resolve problems

MiPevents

• Conferences and Workshops

Bioblast News

• What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..). A term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the Bioblast alert. As Bioblast is updated, the updated ‘article’ can be added to the prepared points for a future Bioblast alert.
• Projects in mitochondrial science (COST HypoxiaNet; Framework programs; ..)
• Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies

Not included in Bioblast alert

MiPNet O2k-Publications

A Bioblast editor may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and ‘present’ the lab on the start page of the Bioblast website.

MiPNet Laboratories of the World

Contact the MiPNet laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects (HypoxiaNet), perhaps add pictures, perhaps keywords/short description. Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..

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