Talk:Azide: Difference between revisions
From Bioblast
No edit summary |
(deleted titration instructions, because they did not change by taking the volume of the solute into account) ย |
||
(2 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
== Former Instructions == | |||
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended. | |||
::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O)ย for '''2-mL O2k-chamber''': | |||
::::# Weigh 260 mg of sodium azide. | |||
::::# Dissolve in 1 mL H<sub>2</sub>0. | |||
::::# Divide into 0.5 mL portions. | |||
::::# Store at -20 ยฐC. | |||
<!-- | |||
[[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]] | [[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]] | ||
== Popular Bioblast page == | == Popular Bioblast page == | ||
::: [[Azide]] has been accessed more than ย | ::: [[Azide]] has been accessed more than ย | ||
Line 6: | Line 20: | ||
:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 gยทmol<sup>-1</sup>. | :::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 gยทmol<sup>-1</sup>. | ||
:::: 2021-02: The preparation instructions were updated to take the volume of the solute into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | :::: 2021-02: The preparation instructions were updated to take the volume of the solute into account (see [[User:Spitzer_Gudrun/Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | ||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ||
Line 33: | Line 47: | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): โฅ25 ยตL using a 100 ยตL Hamilton syringe. | ::::* Titration volume ('''0.5-mL O2k-chamber'''): โฅ25 ยตL using a 100 ยตL Hamilton syringe. | ||
::::* Final concentration: โฅ100 mM. | ::::* Final concentration: โฅ100 mM. | ||
--> |
Latest revision as of 13:04, 8 March 2021
Former Instructions
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
- Preparation of 4 M storage and stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 1 mL H20.
- Divide into 0.5 mL portions.
- Store at -20 ยฐC.