Difference between revisions of "TMPD"

Revision as of 12:02, 22 May 2012

high-resolution terminology - matching measurements at high-resolution

TMPD

Description

N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride is applied as an artificial substrate for reducing cytochrome c in the respirometric assay for cytochrome c oxidase activity. It is maintained in a reduced state by ascorbate and undergoes autooxidation as a function of oxygen pressure, ascorbate and cytochrome c concentration.

Abbreviation: Tm

Reference: MiPNet06.06, MiPNet09.12

MitoPedia topics: Substrate and metabolite

Application in HRR

TMPD: N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride (N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C₁₀H₁₆N₂*2HCl); Sigma T 3134, 5 g, store at R.T.; FW = 237.2

Preparation of 200 mM stock solution (dissolved in H₂O):

1) weigh 47.4 mg of N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride

2) dissolve in 1 ml H₂O

3) add 0.8 M ascorbate to a final concentration of 10 mM

4) divide into 0.2 ml portions

5) store frozen at -20 °C

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 {{MitoPedia
 |abbr=Tm
-|description='''N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride''' is applied as an artificial substrate for reducing cytochrome ''c'' in the respirometric assay for [[Complex IV|cytochrome c oxidase]] activity. It is maintained in a reduced state by [[Ascorbate|ascorbate]] and undergoes autooxidation as a function of oxygen pressure, ascorbate and [[cytochrome c]] concentration.
+|description='''N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride''' is applied as an artificial substrate for reducing cytochrome ''c'' in the respirometric assay for [[Complex IV|cytochrome c oxidase]] activity. It is maintained in a reduced state by [[Ascorbate|ascorbate]] and undergoes autooxidation as a function of [[oxygen pressure]], ascorbate and [[cytochrome c]] concentration.
 |info=MiPNet06.06, MiPNet09.12
 |type=Substrate ETS